Thickness distribution File created on: 2019-07-05 16:25:28 Image name: 1 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - 50% - C=0.ome.tif: A - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 Spots Measured (abs): 21928.00 1653.00 3826.00 9232.00 19280.00 31473.00 52333.00 82456.00 116201.00 130001.00 129353.00 113853.00 94035.00 75219.00 57287.00 42143.00 30808.00 19574.00 10601.00 4607.00 1800.00 634.00 232.00 47.00 Spots Measured (%): 2.09 0.16 0.36 0.88 1.84 3.00 4.99 7.86 11.08 12.40 12.34 10.86 8.97 7.17 5.46 4.02 2.94 1.87 1.01 0.44 0.17 0.06 0.02 0.00 Average thickness (Entire area) (µm): 10.01837 Average thickness (Biomass) (µm): 10.23235 Appended to file on: 2019-07-05 16:25:29 Image name: 10 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - Outlet - C=1.ome.tif: A - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 836678.00 17303.00 19003.00 19730.00 20125.00 20529.00 20350.00 18868.00 16323.00 13014.00 10284.00 8202.00 7550.00 6497.00 4456.00 2827.00 2059.00 1701.00 1330.00 749.00 433.00 242.00 170.00 60.00 58.00 27.00 8.00 Spots Measured (%): 79.79 1.65 1.81 1.88 1.92 1.96 1.94 1.80 1.56 1.24 0.98 0.78 0.72 0.62 0.42 0.27 0.20 0.16 0.13 0.07 0.04 0.02 0.02 0.01 0.01 0.00 0.00 Average thickness (Entire area) (µm): 1.32865 Average thickness (Biomass) (µm): 6.57483 Appended to file on: 2019-07-05 16:25:31 Image name: 11 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - Inlet - C=0.ome.tif: B - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 Spots Measured (abs): 17340.00 183.00 180.00 300.00 544.00 1069.00 2089.00 3741.00 6918.00 11957.00 19533.00 28980.00 39480.00 46277.00 55847.00 64016.00 73954.00 81727.00 82056.00 79522.00 72907.00 64714.00 59553.00 52690.00 46323.00 39093.00 31077.00 22842.00 15818.00 10454.00 6987.00 4604.00 3294.00 2507.00 Spots Measured (%): 1.65 0.02 0.02 0.03 0.05 0.10 0.20 0.36 0.66 1.14 1.86 2.76 3.77 4.41 5.33 6.11 7.05 7.79 7.83 7.58 6.95 6.17 5.68 5.02 4.42 3.73 2.96 2.18 1.51 1.00 0.67 0.44 0.31 0.24 Average thickness (Entire area) (µm): 18.34537 Average thickness (Biomass) (µm): 18.65384 Appended to file on: 2019-07-05 16:25:33 Image name: 12 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - Inlet - C=1.ome.tif: B - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 Spots Measured (abs): 383357.00 1861.00 2029.00 2782.00 4130.00 5863.00 8282.00 11942.00 17294.00 25263.00 36411.00 48315.00 60615.00 65973.00 64819.00 58878.00 53579.00 46430.00 37534.00 30627.00 22240.00 16105.00 12025.00 9038.00 6889.00 5330.00 3964.00 2991.00 1707.00 1011.00 565.00 343.00 256.00 128.00 Spots Measured (%): 36.56 0.18 0.19 0.27 0.39 0.56 0.79 1.14 1.65 2.41 3.47 4.61 5.78 6.29 6.18 5.62 5.11 4.43 3.58 2.92 2.12 1.54 1.15 0.86 0.66 0.51 0.38 0.29 0.16 0.10 0.05 0.03 0.02 0.01 Average thickness (Entire area) (µm): 9.14242 Average thickness (Biomass) (µm): 14.41107 Appended to file on: 2019-07-05 16:25:34 Image name: 13 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - 25% - C=0.ome.tif: B - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.00 31.00 671.00 6091.00 27693.00 65424.00 112370.00 146033.00 158567.00 147026.00 125729.00 95305.00 66792.00 43962.00 27632.00 14786.00 7733.00 2235.00 367.00 91.00 22.00 11.00 3.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.06 0.58 2.64 6.24 10.72 13.93 15.12 14.02 11.99 9.09 6.37 4.19 2.64 1.41 0.74 0.21 0.03 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 18.77184 Average thickness (Biomass) (µm): 18.77184 Appended to file on: 2019-07-05 16:25:36 Image name: 14 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - 25% - C=1.ome.tif: B - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 94561.00 393.00 418.00 734.00 1351.00 2406.00 4434.00 7297.00 11829.00 17665.00 25699.00 36124.00 50803.00 68893.00 88581.00 96365.00 101120.00 98033.00 88057.00 72451.00 58038.00 43781.00 30736.00 20328.00 13086.00 7551.00 4251.00 2272.00 828.00 273.00 121.00 66.00 31.00 Spots Measured (%): 9.02 0.04 0.04 0.07 0.13 0.23 0.42 0.70 1.13 1.68 2.45 3.45 4.84 6.57 8.45 9.19 9.64 9.35 8.40 6.91 5.53 4.18 2.93 1.94 1.25 0.72 0.41 0.22 0.08 0.03 0.01 0.01 0.00 Average thickness (Entire area) (µm): 14.66294 Average thickness (Biomass) (µm): 16.11632 Appended to file on: 2019-07-05 16:25:37 Image name: 15 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - 50% - C=0.ome.tif: B - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 300034.00 52407.00 56127.00 61332.00 68900.00 73711.00 75904.00 74090.00 68132.00 59012.00 49234.00 37759.00 25705.00 16886.00 10972.00 7576.00 4686.00 3118.00 1948.00 692.00 292.00 56.00 3.00 0.00 0.00 0.00 0.00 Spots Measured (%): 28.61 5.00 5.35 5.85 6.57 7.03 7.24 7.07 6.50 5.63 4.70 3.60 2.45 1.61 1.05 0.72 0.45 0.30 0.19 0.07 0.03 0.01 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 4.69946 Average thickness (Biomass) (µm): 6.58312 Appended to file on: 2019-07-05 16:25:38 Image name: 16 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - 50% - C=1.ome.tif: B - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 954169.00 9042.00 9385.00 9697.00 10341.00 10359.00 9512.00 8377.00 6698.00 5342.00 4617.00 3976.00 2939.00 1860.00 1056.00 658.00 289.00 146.00 81.00 32.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 91.00 0.86 0.90 0.92 0.99 0.99 0.91 0.80 0.64 0.51 0.44 0.38 0.28 0.18 0.10 0.06 0.03 0.01 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.52495 Average thickness (Biomass) (µm): 5.83060 Appended to file on: 2019-07-05 16:25:40 Image name: 17 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - 75% - C=0.ome.tif: B - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 Spots Measured (abs): 2605.00 113.00 329.00 802.00 1721.00 4203.00 9201.00 19393.00 36501.00 59605.00 88733.00 117120.00 136496.00 138579.00 114408.00 81850.00 56236.00 40349.00 37043.00 31117.00 25769.00 19007.00 13630.00 8066.00 3646.00 1542.00 435.00 72.00 5.00 0.00 Spots Measured (%): 0.25 0.01 0.03 0.08 0.16 0.40 0.88 1.85 3.48 5.68 8.46 11.17 13.02 13.22 10.91 7.81 5.36 3.85 3.53 2.97 2.46 1.81 1.30 0.77 0.35 0.15 0.04 0.01 0.00 0.00 Average thickness (Entire area) (µm): 13.23125 Average thickness (Biomass) (µm): 13.26420 Appended to file on: 2019-07-05 16:25:41 Image name: 18 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - 75% - C=1.ome.tif: B - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 Spots Measured (abs): 639534.00 6377.00 9473.00 13368.00 17249.00 20719.00 23681.00 26749.00 29847.00 32535.00 35569.00 36377.00 34746.00 30909.00 25624.00 19130.00 14625.00 9768.00 7331.00 4949.00 3823.00 2551.00 1730.00 1028.00 490.00 198.00 89.00 49.00 44.00 14.00 Spots Measured (%): 60.99 0.61 0.90 1.27 1.64 1.98 2.26 2.55 2.85 3.10 3.39 3.47 3.31 2.95 2.44 1.82 1.39 0.93 0.70 0.47 0.36 0.24 0.16 0.10 0.05 0.02 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 3.96514 Average thickness (Biomass) (µm): 10.16460 Appended to file on: 2019-07-05 16:25:43 Image name: 19 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - Outlet - C=0.ome.tif: B - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 6613.00 227.00 494.00 1164.00 2702.00 6214.00 13547.00 26489.00 47110.00 70460.00 94260.00 114508.00 121455.00 108758.00 86650.00 71208.00 58713.00 48374.00 37886.00 30678.00 24055.00 18554.00 15042.00 11432.00 8312.00 6731.00 5021.00 4020.00 3299.00 2536.00 1528.00 476.00 60.00 Spots Measured (%): 0.63 0.02 0.05 0.11 0.26 0.59 1.29 2.53 4.49 6.72 8.99 10.92 11.58 10.37 8.26 6.79 5.60 4.61 3.61 2.93 2.29 1.77 1.43 1.09 0.79 0.64 0.48 0.38 0.31 0.24 0.15 0.05 0.01 Average thickness (Entire area) (µm): 13.35268 Average thickness (Biomass) (µm): 13.43743 Appended to file on: 2019-07-05 16:25:44 Image name: 2 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - 50% - C=1.ome.tif: A - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 Spots Measured (abs): 970573.00 3099.00 4924.00 7109.00 10320.00 7277.00 7842.00 7675.00 7322.00 5506.00 4352.00 3445.00 2677.00 2077.00 1628.00 1054.00 865.00 514.00 286.00 31.00 0.00 0.00 0.00 0.00 Spots Measured (%): 92.56 0.30 0.47 0.68 0.98 0.69 0.75 0.73 0.70 0.53 0.42 0.33 0.26 0.20 0.16 0.10 0.08 0.05 0.03 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.50368 Average thickness (Biomass) (µm): 6.77082 Appended to file on: 2019-07-05 16:25:45 Image name: 20 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - B - Outlet - C=1.ome.tif: B - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 570268.00 7120.00 11269.00 16941.00 22453.00 27249.00 29947.00 33379.00 36172.00 40447.00 43004.00 42286.00 36878.00 28714.00 21896.00 17535.00 14465.00 12163.00 9572.00 7503.00 5531.00 3857.00 3091.00 2134.00 1470.00 1005.00 619.00 531.00 430.00 293.00 142.00 97.00 115.00 Spots Measured (%): 54.38 0.68 1.07 1.62 2.14 2.60 2.86 3.18 3.45 3.86 4.10 4.03 3.52 2.74 2.09 1.67 1.38 1.16 0.91 0.72 0.53 0.37 0.29 0.20 0.14 0.10 0.06 0.05 0.04 0.03 0.01 0.01 0.01 Average thickness (Entire area) (µm): 4.61243 Average thickness (Biomass) (µm): 10.11164 Appended to file on: 2019-07-05 16:25:47 Image name: 3 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - 25% - C=0.ome.tif: A - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 Spots Measured (abs): 405.00 0.00 2.00 2.00 16.00 31.00 132.00 563.00 1931.00 5474.00 13384.00 26952.00 45164.00 61993.00 74456.00 81239.00 86945.00 85875.00 84718.00 83006.00 76194.00 64882.00 50766.00 39170.00 30098.00 21902.00 18504.00 17487.00 16748.00 16342.00 13748.00 10719.00 7281.00 4103.00 2480.00 1551.00 1081.00 1113.00 989.00 707.00 423.00 Spots Measured (%): 0.04 0.00 0.00 0.00 0.00 0.00 0.01 0.05 0.18 0.52 1.28 2.57 4.31 5.91 7.10 7.75 8.29 8.19 8.08 7.92 7.27 6.19 4.84 3.74 2.87 2.09 1.76 1.67 1.60 1.56 1.31 1.02 0.69 0.39 0.24 0.15 0.10 0.11 0.09 0.07 0.04 Average thickness (Entire area) (µm): 18.63487 Average thickness (Biomass) (µm): 18.64207 Appended to file on: 2019-07-05 16:25:49 Image name: 4 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - 25% - C=1.ome.tif: A - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 Spots Measured (abs): 219700.00 2724.00 3922.00 6394.00 9516.00 13601.00 18453.00 24807.00 33408.00 43373.00 55818.00 69470.00 78462.00 81890.00 75845.00 65088.00 54680.00 43615.00 34872.00 27719.00 19847.00 13985.00 9509.00 6697.00 4793.00 3032.00 2753.00 2853.00 3159.00 3607.00 3047.00 2161.00 1736.00 1271.00 1286.00 1092.00 905.00 1035.00 1265.00 904.00 282.00 Spots Measured (%): 20.95 0.26 0.37 0.61 0.91 1.30 1.76 2.37 3.19 4.14 5.32 6.63 7.48 7.81 7.23 6.21 5.21 4.16 3.33 2.64 1.89 1.33 0.91 0.64 0.46 0.29 0.26 0.27 0.30 0.34 0.29 0.21 0.17 0.12 0.12 0.10 0.09 0.10 0.12 0.09 0.03 Average thickness (Entire area) (µm): 10.77785 Average thickness (Biomass) (µm): 13.63460 Appended to file on: 2019-07-05 16:25:51 Image name: 5 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - Inlet - C=0.ome.tif: A - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 Spots Measured (abs): 2760.00 612.00 1038.00 1894.00 3486.00 6570.00 11492.00 19005.00 29021.00 40350.00 52596.00 66271.00 82017.00 95866.00 104050.00 106090.00 99110.00 86313.00 68565.00 51810.00 39485.00 27833.00 19957.00 14138.00 9633.00 5198.00 2232.00 867.00 317.00 Spots Measured (%): 0.26 0.06 0.10 0.18 0.33 0.63 1.10 1.81 2.77 3.85 5.02 6.32 7.82 9.14 9.92 10.12 9.45 8.23 6.54 4.94 3.77 2.65 1.90 1.35 0.92 0.50 0.21 0.08 0.03 Average thickness (Entire area) (µm): 14.53673 Average thickness (Biomass) (µm): 14.57510 Appended to file on: 2019-07-05 16:25:52 Image name: 6 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - Inlet - C=1.ome.tif: A - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 Spots Measured (abs): 658367.00 7601.00 8718.00 10497.00 12866.00 15244.00 17833.00 20795.00 23647.00 26901.00 29306.00 31594.00 32755.00 30620.00 27616.00 23546.00 19069.00 15455.00 11440.00 8415.00 6388.00 4026.00 2418.00 1443.00 1002.00 553.00 289.00 87.00 85.00 Spots Measured (%): 62.79 0.72 0.83 1.00 1.23 1.45 1.70 1.98 2.26 2.57 2.79 3.01 3.12 2.92 2.63 2.25 1.82 1.47 1.09 0.80 0.61 0.38 0.23 0.14 0.10 0.05 0.03 0.01 0.01 Average thickness (Entire area) (µm): 4.12058 Average thickness (Biomass) (µm): 11.07289 Appended to file on: 2019-07-05 16:25:53 Image name: 7 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - 75% - C=0.ome.tif: A - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 Spots Measured (abs): 54759.00 10178.00 15813.00 23737.00 34360.00 47093.00 63663.00 82723.00 100521.00 110559.00 106387.00 94361.00 79241.00 64476.00 49918.00 36311.00 26598.00 18838.00 12327.00 7561.00 4667.00 2536.00 1308.00 474.00 121.00 18.00 17.00 11.00 Spots Measured (%): 5.22 0.97 1.51 2.26 3.28 4.49 6.07 7.89 9.59 10.54 10.15 9.00 7.56 6.15 4.76 3.46 2.54 1.80 1.18 0.72 0.45 0.24 0.12 0.05 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 9.23101 Average thickness (Biomass) (µm): 9.73964 Appended to file on: 2019-07-05 16:25:54 Image name: 8 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - 75% - C=1.ome.tif: A - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 Spots Measured (abs): 814240.00 14080.00 16657.00 18014.00 18818.00 18873.00 19977.00 19822.00 19043.00 17419.00 15129.00 12348.00 10250.00 8185.00 6860.00 5465.00 4379.00 3257.00 2183.00 1433.00 963.00 464.00 338.00 233.00 88.00 42.00 16.00 0.00 Spots Measured (%): 77.65 1.34 1.59 1.72 1.79 1.80 1.91 1.89 1.82 1.66 1.44 1.18 0.98 0.78 0.65 0.52 0.42 0.31 0.21 0.14 0.09 0.04 0.03 0.02 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 1.68814 Average thickness (Biomass) (µm): 7.55386 Appended to file on: 2019-07-05 16:25:56 Image name: 9 - Ray Allan 72h PAO1 WT microfluidics 05.07.19.lif - A - Outlet - C=0.ome.tif: A - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 338637.00 58629.00 66042.00 69733.00 70111.00 65724.00 60825.00 54320.00 47946.00 40333.00 34767.00 28994.00 25581.00 20691.00 16152.00 13321.00 10985.00 8797.00 6632.00 4593.00 2885.00 1573.00 783.00 386.00 89.00 30.00 17.00 Spots Measured (%): 32.29 5.59 6.30 6.65 6.69 6.27 5.80 5.18 4.57 3.85 3.32 2.77 2.44 1.97 1.54 1.27 1.05 0.84 0.63 0.44 0.28 0.15 0.07 0.04 0.01 0.00 0.00 Average thickness (Entire area) (µm): 4.59662 Average thickness (Biomass) (µm): 6.78918