Thickness distribution File created on: 2019-07-05 17:38:21 Image name: 1 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - Inlet - C=0.ome.tif: Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 51.98 52.98 Spots Measured (abs): 1045830.00 2569.00 165.00 11.00 1.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 99.74 0.24 0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.00280 Average thickness (Biomass) (µm): 1.06887 Appended to file on: 2019-07-05 17:38:26 Image name: 10 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - Outlet - C=1.ome.tif: Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 Spots Measured (abs): 503.00 45.00 23.00 31.00 44.00 70.00 143.00 228.00 375.00 801.00 1664.00 3694.00 8183.00 17723.00 34129.00 58493.00 86709.00 112947.00 123052.00 120941.00 104554.00 91001.00 74396.00 56004.00 40783.00 29451.00 21417.00 13733.00 9462.00 7962.00 7174.00 6369.00 5476.00 4231.00 3305.00 3460.00 Spots Measured (%): 0.05 0.00 0.00 0.00 0.00 0.01 0.01 0.02 0.04 0.08 0.16 0.35 0.78 1.69 3.25 5.58 8.27 10.77 11.74 11.53 9.97 8.68 7.09 5.34 3.89 2.81 2.04 1.31 0.90 0.76 0.68 0.61 0.52 0.40 0.32 0.33 Average thickness (Entire area) (µm): 19.64087 Average thickness (Biomass) (µm): 19.65030 Appended to file on: 2019-07-05 17:38:28 Image name: 2 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - Inlet - C=1.ome.tif: Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 51.98 52.98 Spots Measured (abs): 159.00 57.00 102.00 168.00 361.00 722.00 1708.00 3408.00 6937.00 11959.00 19157.00 27362.00 35153.00 39417.00 45262.00 48806.00 53390.00 54280.00 54767.00 55389.00 52231.00 51974.00 50167.00 47172.00 46308.00 45540.00 40702.00 37423.00 33494.00 27986.00 23422.00 20127.00 19421.00 17776.00 16244.00 14053.00 12795.00 10243.00 7284.00 5131.00 3830.00 2989.00 1947.00 1168.00 464.00 110.00 11.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 0.02 0.01 0.01 0.02 0.03 0.07 0.16 0.33 0.66 1.14 1.83 2.61 3.35 3.76 4.32 4.65 5.09 5.18 5.22 5.28 4.98 4.96 4.78 4.50 4.42 4.34 3.88 3.57 3.19 2.67 2.23 1.92 1.85 1.70 1.55 1.34 1.22 0.98 0.69 0.49 0.37 0.29 0.19 0.11 0.04 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 21.48219 Average thickness (Biomass) (µm): 21.48545 Appended to file on: 2019-07-05 17:38:30 Image name: 3 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - 25% - C=0.ome.tif: 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 Spots Measured (abs): 1045094.00 3208.00 252.00 20.00 2.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 99.67 0.31 0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.00360 Average thickness (Biomass) (µm): 1.08526 Appended to file on: 2019-07-05 17:38:33 Image name: 4 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - 25% - C=1.ome.tif: 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 Spots Measured (abs): 40.00 25.00 51.00 82.00 155.00 316.00 576.00 1122.00 1895.00 2858.00 4158.00 5475.00 7062.00 8916.00 11531.00 14695.00 19438.00 24911.00 29822.00 33904.00 39398.00 45885.00 51453.00 55379.00 61997.00 67719.00 68623.00 64532.00 60497.00 52720.00 47779.00 43479.00 40849.00 34295.00 29839.00 23223.00 17686.00 14536.00 12181.00 10647.00 8977.00 8171.00 7211.00 4988.00 3700.00 2780.00 1586.00 870.00 544.00 Spots Measured (%): 0.00 0.00 0.00 0.01 0.01 0.03 0.05 0.11 0.18 0.27 0.40 0.52 0.67 0.85 1.10 1.40 1.85 2.38 2.84 3.23 3.76 4.38 4.91 5.28 5.91 6.46 6.54 6.15 5.77 5.03 4.56 4.15 3.90 3.27 2.85 2.21 1.69 1.39 1.16 1.02 0.86 0.78 0.69 0.48 0.35 0.27 0.15 0.08 0.05 Average thickness (Entire area) (µm): 26.20047 Average thickness (Biomass) (µm): 26.20147 Appended to file on: 2019-07-05 17:38:34 Image name: 5 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - 50% - C=0.ome.tif: 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 Spots Measured (abs): 1041561.00 6243.00 690.00 75.00 7.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 99.33 0.60 0.07 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.00751 Average thickness (Biomass) (µm): 1.12240 Appended to file on: 2019-07-05 17:38:36 Image name: 6 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - 50% - C=1.ome.tif: 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 Spots Measured (abs): 33.00 17.00 16.00 31.00 52.00 139.00 202.00 572.00 1088.00 2452.00 4901.00 9269.00 15585.00 21908.00 28634.00 36854.00 40438.00 44463.00 45633.00 46236.00 47224.00 50133.00 55077.00 61523.00 67749.00 73009.00 71195.00 69007.00 63034.00 54741.00 44735.00 34698.00 24756.00 17774.00 10295.00 3918.00 995.00 183.00 7.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.01 0.02 0.05 0.10 0.23 0.47 0.88 1.49 2.09 2.73 3.51 3.86 4.24 4.35 4.41 4.50 4.78 5.25 5.87 6.46 6.96 6.79 6.58 6.01 5.22 4.27 3.31 2.36 1.70 0.98 0.37 0.09 0.02 0.00 Average thickness (Entire area) (µm): 23.05732 Average thickness (Biomass) (µm): 23.05804 Appended to file on: 2019-07-05 17:38:37 Image name: 7 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - 75% - C=0.ome.tif: 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 1041483.00 6260.00 732.00 91.00 9.00 1.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 99.32 0.60 0.07 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.00766 Average thickness (Biomass) (µm): 1.13289 Appended to file on: 2019-07-05 17:38:39 Image name: 8 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - 75% - C=1.ome.tif: 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 671.00 121.00 155.00 265.00 542.00 971.00 1667.00 3105.00 5995.00 10545.00 16836.00 26256.00 39501.00 51712.00 67025.00 79267.00 88523.00 86207.00 83058.00 77897.00 71256.00 65607.00 60573.00 52963.00 45301.00 38448.00 28565.00 20280.00 13519.00 7605.00 2975.00 932.00 172.00 42.00 19.00 Spots Measured (%): 0.06 0.01 0.01 0.03 0.05 0.09 0.16 0.30 0.57 1.01 1.61 2.50 3.77 4.93 6.39 7.56 8.44 8.22 7.92 7.43 6.80 6.26 5.78 5.05 4.32 3.67 2.72 1.93 1.29 0.73 0.28 0.09 0.02 0.00 0.00 Average thickness (Entire area) (µm): 18.29330 Average thickness (Biomass) (µm): 18.30502 Appended to file on: 2019-07-05 17:38:41 Image name: 9 - Ray Allan 72h PAO1 WT microfluidics 7.1.19.lif - Outlet - C=0.ome.tif: Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 Spots Measured (abs): 1037142.00 9615.00 1403.00 247.00 61.00 51.00 21.00 23.00 8.00 0.00 1.00 4.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 98.91 0.92 0.13 0.02 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.01341 Average thickness (Biomass) (µm): 1.22982