Thickness distribution File created on: 2019-07-05 17:19:34 Image name: 1 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT Inlet - C=0.ome.tif: PA3352 Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 Spots Measured (abs): 0.00 0.00 0.00 0.00 2.00 0.00 4.00 9.00 46.00 147.00 385.00 1114.00 2663.00 5909.00 10100.00 15826.00 23384.00 33924.00 43789.00 50578.00 53804.00 57284.00 57195.00 56804.00 55610.00 55999.00 58399.00 56959.00 53718.00 50476.00 46200.00 42693.00 40004.00 39645.00 35618.00 29076.00 19964.00 13490.00 10359.00 8862.00 7242.00 5447.00 3010.00 1980.00 858.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.04 0.11 0.25 0.56 0.96 1.51 2.23 3.24 4.18 4.82 5.13 5.46 5.45 5.42 5.30 5.34 5.57 5.43 5.12 4.81 4.41 4.07 3.82 3.78 3.40 2.77 1.90 1.29 0.99 0.85 0.69 0.52 0.29 0.19 0.08 Average thickness (Entire area) (µm): 25.79305 Average thickness (Biomass) (µm): 25.79305 Appended to file on: 2019-07-05 17:19:36 Image name: 10 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 75% - C=0.ome.tif: PA0996 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 12360.00 581.00 1278.00 2443.00 4447.00 6778.00 10229.00 14465.00 18774.00 22648.00 24991.00 27745.00 30336.00 32935.00 35861.00 39371.00 44194.00 49708.00 57944.00 63328.00 71391.00 80567.00 82846.00 76619.00 70689.00 62419.00 48336.00 28608.00 14026.00 4991.00 2728.00 2450.00 2490.00 Spots Measured (%): 1.18 0.06 0.12 0.23 0.42 0.65 0.98 1.38 1.79 2.16 2.38 2.65 2.89 3.14 3.42 3.75 4.21 4.74 5.53 6.04 6.81 7.68 7.90 7.31 6.74 5.95 4.61 2.73 1.34 0.48 0.26 0.23 0.24 Average thickness (Entire area) (µm): 18.66996 Average thickness (Biomass) (µm): 18.89266 Appended to file on: 2019-07-05 17:19:38 Image name: 11 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 75% - C=1.ome.tif: PA0996 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 43.00 2.00 6.00 12.00 20.00 76.00 145.00 401.00 997.00 2542.00 5909.00 13195.00 24069.00 39175.00 56866.00 74978.00 91053.00 102311.00 111736.00 113238.00 110957.00 98506.00 78218.00 53850.00 33150.00 17849.00 8983.00 4448.00 2681.00 1754.00 1010.00 340.00 56.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.01 0.01 0.04 0.10 0.24 0.56 1.26 2.30 3.74 5.42 7.15 8.68 9.76 10.66 10.80 10.58 9.39 7.46 5.14 3.16 1.70 0.86 0.42 0.26 0.17 0.10 0.03 0.01 Average thickness (Entire area) (µm): 18.42731 Average thickness (Biomass) (µm): 18.42807 Appended to file on: 2019-07-05 17:19:40 Image name: 12 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 Outlet - C=0.ome.tif: PA0996 Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 Spots Measured (abs): 3.00 0.00 0.00 3.00 5.00 20.00 35.00 131.00 474.00 1532.00 3959.00 7937.00 13262.00 19133.00 26791.00 34000.00 43528.00 52590.00 57759.00 62596.00 67733.00 69821.00 73904.00 78035.00 83454.00 79211.00 67543.00 53225.00 44710.00 34650.00 26953.00 19743.00 13352.00 7859.00 3546.00 872.00 164.00 43.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.05 0.15 0.38 0.76 1.26 1.82 2.55 3.24 4.15 5.02 5.51 5.97 6.46 6.66 7.05 7.44 7.96 7.55 6.44 5.08 4.26 3.30 2.57 1.88 1.27 0.75 0.34 0.08 0.02 0.00 0.00 Average thickness (Entire area) (µm): 22.11731 Average thickness (Biomass) (µm): 22.11738 Appended to file on: 2019-07-05 17:19:43 Image name: 13 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 Outlet - C=1.ome.tif: PA0996 Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 Spots Measured (abs): 5.00 0.00 0.00 2.00 5.00 8.00 10.00 24.00 76.00 281.00 787.00 2170.00 5201.00 9834.00 17787.00 29360.00 43653.00 58272.00 70854.00 81523.00 88788.00 90517.00 91527.00 90685.00 84111.00 71958.00 58232.00 44991.00 35039.00 25193.00 18303.00 13599.00 8588.00 4479.00 1658.00 612.00 305.00 88.00 51.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.08 0.21 0.50 0.94 1.70 2.80 4.16 5.56 6.76 7.77 8.47 8.63 8.73 8.65 8.02 6.86 5.55 4.29 3.34 2.40 1.75 1.30 0.82 0.43 0.16 0.06 0.03 0.01 0.00 Average thickness (Entire area) (µm): 21.87981 Average thickness (Biomass) (µm): 21.87992 Appended to file on: 2019-07-05 17:19:45 Image name: 14 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 25% - C=0.ome.tif: PA0996 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 Spots Measured (abs): 9812.00 71.00 133.00 223.00 576.00 1598.00 3839.00 7856.00 12474.00 14642.00 13818.00 12005.00 10629.00 10274.00 9677.00 9205.00 9141.00 9189.00 9340.00 10409.00 12155.00 14915.00 17588.00 21802.00 26865.00 35270.00 48649.00 62333.00 77980.00 95155.00 99543.00 102875.00 91712.00 74543.00 52101.00 30967.00 16683.00 8527.00 3241.00 761.00 Spots Measured (%): 0.94 0.01 0.01 0.02 0.05 0.15 0.37 0.75 1.19 1.40 1.32 1.14 1.01 0.98 0.92 0.88 0.87 0.88 0.89 0.99 1.16 1.42 1.68 2.08 2.56 3.36 4.64 5.94 7.44 9.07 9.49 9.81 8.75 7.11 4.97 2.95 1.59 0.81 0.31 0.07 Average thickness (Entire area) (µm): 26.83834 Average thickness (Biomass) (µm): 27.09185 Appended to file on: 2019-07-05 17:19:47 Image name: 15 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 25% - C=1.ome.tif: PA0996 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 Spots Measured (abs): 275754.00 1056.00 1415.00 2512.00 4455.00 7565.00 12267.00 18170.00 25227.00 32194.00 36192.00 37395.00 36005.00 36014.00 34985.00 33920.00 33758.00 33236.00 31752.00 30607.00 29222.00 28632.00 28023.00 27464.00 26806.00 25666.00 24861.00 22722.00 20942.00 18576.00 16053.00 13532.00 11506.00 9172.00 7118.00 4884.00 3358.00 2464.00 1516.00 1580.00 Spots Measured (%): 26.30 0.10 0.13 0.24 0.42 0.72 1.17 1.73 2.41 3.07 3.45 3.57 3.43 3.43 3.34 3.23 3.22 3.17 3.03 2.92 2.79 2.73 2.67 2.62 2.56 2.45 2.37 2.17 2.00 1.77 1.53 1.29 1.10 0.87 0.68 0.47 0.32 0.23 0.14 0.15 Average thickness (Entire area) (µm): 13.45208 Average thickness (Biomass) (µm): 18.25197 Appended to file on: 2019-07-05 17:19:49 Image name: 16 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA1084 50% - C=0.ome.tif: PA1084 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 49036.00 2978.00 4586.00 7034.00 10478.00 15222.00 22075.00 30727.00 42145.00 56420.00 73194.00 88524.00 99935.00 104471.00 101965.00 95316.00 82612.00 65088.00 44852.00 26406.00 14384.00 7057.00 3101.00 801.00 144.00 15.00 10.00 Spots Measured (%): 4.68 0.28 0.44 0.67 1.00 1.45 2.11 2.93 4.02 5.38 6.98 8.44 9.53 9.96 9.72 9.09 7.88 6.21 4.28 2.52 1.37 0.67 0.30 0.08 0.01 0.00 0.00 Average thickness (Entire area) (µm): 12.13473 Average thickness (Biomass) (µm): 12.73004 Appended to file on: 2019-07-05 17:19:50 Image name: 17 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA1084 50% - C=1.ome.tif: PA1084 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 834831.00 2719.00 3258.00 3319.00 2834.00 2328.00 2118.00 2509.00 3218.00 4935.00 8139.00 12704.00 17864.00 22209.00 24092.00 23507.00 21175.00 18547.00 13892.00 9705.00 5847.00 3654.00 2261.00 1416.00 773.00 433.00 289.00 Spots Measured (%): 79.62 0.26 0.31 0.32 0.27 0.22 0.20 0.24 0.31 0.47 0.78 1.21 1.70 2.12 2.30 2.24 2.02 1.77 1.32 0.93 0.56 0.35 0.22 0.14 0.07 0.04 0.03 Average thickness (Entire area) (µm): 2.82025 Average thickness (Biomass) (µm): 13.83540 Appended to file on: 2019-07-05 17:19:52 Image name: 18 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA1084 75% - C=0.ome.tif: PA1084 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 74929.00 41.00 57.00 297.00 1057.00 2569.00 4773.00 8184.00 14252.00 26458.00 52276.00 86958.00 115361.00 121638.00 104636.00 79071.00 61245.00 44380.00 31193.00 24458.00 23492.00 24919.00 26410.00 26807.00 23023.00 18628.00 16019.00 13179.00 10008.00 6898.00 3339.00 1420.00 493.00 95.00 13.00 Spots Measured (%): 7.15 0.00 0.01 0.03 0.10 0.24 0.46 0.78 1.36 2.52 4.99 8.29 11.00 11.60 9.98 7.54 5.84 4.23 2.97 2.33 2.24 2.38 2.52 2.56 2.20 1.78 1.53 1.26 0.95 0.66 0.32 0.14 0.05 0.01 0.00 Average thickness (Entire area) (µm): 14.24147 Average thickness (Biomass) (µm): 15.33745 Appended to file on: 2019-07-05 17:19:54 Image name: 19 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA1084 75% - C=1.ome.tif: PA1084 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 78857.00 172.00 336.00 616.00 1267.00 2360.00 3870.00 6039.00 9511.00 15633.00 28196.00 50019.00 82020.00 113654.00 129595.00 116504.00 90281.00 63729.00 44271.00 32381.00 26745.00 26092.00 25080.00 24557.00 20801.00 16865.00 13260.00 9807.00 6756.00 4516.00 2600.00 1509.00 502.00 104.00 71.00 Spots Measured (%): 7.52 0.02 0.03 0.06 0.12 0.23 0.37 0.58 0.91 1.49 2.69 4.77 7.82 10.84 12.36 11.11 8.61 6.08 4.22 3.09 2.55 2.49 2.39 2.34 1.98 1.61 1.26 0.94 0.64 0.43 0.25 0.14 0.05 0.01 0.01 Average thickness (Entire area) (µm): 14.58137 Average thickness (Biomass) (µm): 15.76712 Appended to file on: 2019-07-05 17:19:56 Image name: 2 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT Inlet - C=0.ome.tif: PAO1 WT Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 0.00 3.00 75.00 687.00 2324.00 4884.00 8326.00 10847.00 13544.00 15487.00 20056.00 26260.00 35517.00 47736.00 59711.00 71589.00 87048.00 99883.00 103601.00 94471.00 81007.00 68314.00 56870.00 42446.00 31168.00 24000.00 18504.00 11160.00 6535.00 3892.00 2630.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.07 0.22 0.47 0.79 1.03 1.29 1.48 1.91 2.50 3.39 4.55 5.69 6.83 8.30 9.53 9.88 9.01 7.73 6.51 5.42 4.05 2.97 2.29 1.76 1.06 0.62 0.37 0.25 Average thickness (Entire area) (µm): 27.53306 Average thickness (Biomass) (µm): 27.53306 Appended to file on: 2019-07-05 17:19:59 Image name: 20 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA1084 Outlet - C=0.ome.tif: PA1084 Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 Spots Measured (abs): 422.00 178.00 297.00 602.00 1180.00 2463.00 4334.00 7837.00 11975.00 17394.00 24091.00 32692.00 42727.00 52286.00 58459.00 62841.00 64952.00 65275.00 65459.00 66237.00 65119.00 63151.00 58949.00 54117.00 47175.00 40411.00 32988.00 26944.00 20577.00 16119.00 12303.00 8472.00 5532.00 3964.00 3410.00 2894.00 2220.00 1462.00 823.00 245.00 Spots Measured (%): 0.04 0.02 0.03 0.06 0.11 0.23 0.41 0.75 1.14 1.66 2.30 3.12 4.07 4.99 5.58 5.99 6.19 6.23 6.24 6.32 6.21 6.02 5.62 5.16 4.50 3.85 3.15 2.57 1.96 1.54 1.17 0.81 0.53 0.38 0.33 0.28 0.21 0.14 0.08 0.02 Average thickness (Entire area) (µm): 18.84420 Average thickness (Biomass) (µm): 18.85179 Appended to file on: 2019-07-05 17:20:01 Image name: 21 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA1084 Outlet - C=1.ome.tif: PA1084 Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 Spots Measured (abs): 7758.00 361.00 492.00 724.00 1093.00 1849.00 3189.00 5464.00 9019.00 14094.00 20236.00 27362.00 35841.00 45411.00 51615.00 56535.00 59884.00 61446.00 64192.00 67243.00 66477.00 64331.00 60402.00 56857.00 51825.00 44283.00 37279.00 32060.00 25767.00 20138.00 15112.00 11329.00 8071.00 5728.00 4289.00 3773.00 3082.00 2006.00 1263.00 696.00 Spots Measured (%): 0.74 0.03 0.05 0.07 0.10 0.18 0.30 0.52 0.86 1.34 1.93 2.61 3.42 4.33 4.92 5.39 5.71 5.86 6.12 6.41 6.34 6.14 5.76 5.42 4.94 4.22 3.56 3.06 2.46 1.92 1.44 1.08 0.77 0.55 0.41 0.36 0.29 0.19 0.12 0.07 Average thickness (Entire area) (µm): 19.45107 Average thickness (Biomass) (µm): 19.59605 Appended to file on: 2019-07-05 17:20:04 Image name: 22 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT 50% - C=0.ome.tif: PAO1 WT 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 Spots Measured (abs): 15832.00 44.00 54.00 142.00 329.00 717.00 1448.00 2892.00 5177.00 9545.00 16370.00 27728.00 42150.00 54786.00 64710.00 71598.00 74265.00 72684.00 69503.00 64674.00 57344.00 49412.00 41183.00 37071.00 34110.00 34772.00 36281.00 37150.00 34870.00 27621.00 21426.00 15410.00 10625.00 7192.00 4741.00 2887.00 1420.00 394.00 18.00 1.00 0.00 0.00 Spots Measured (%): 1.51 0.00 0.01 0.01 0.03 0.07 0.14 0.28 0.49 0.91 1.56 2.64 4.02 5.22 6.17 6.83 7.08 6.93 6.63 6.17 5.47 4.71 3.93 3.54 3.25 3.32 3.46 3.54 3.33 2.63 2.04 1.47 1.01 0.69 0.45 0.28 0.14 0.04 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 19.17591 Average thickness (Biomass) (µm): 19.46988 Appended to file on: 2019-07-05 17:20:05 Image name: 23 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT 50% - C=1.ome.tif: PAO1 WT 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 Spots Measured (abs): 730090.00 2962.00 3873.00 5413.00 7192.00 9613.00 11184.00 13144.00 14109.00 15270.00 16353.00 17625.00 19742.00 21608.00 22630.00 23112.00 22067.00 19833.00 17392.00 15192.00 12452.00 9163.00 6595.00 4689.00 2788.00 1698.00 1058.00 621.00 395.00 217.00 191.00 145.00 89.00 42.00 29.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 69.63 0.28 0.37 0.52 0.69 0.92 1.07 1.25 1.35 1.46 1.56 1.68 1.88 2.06 2.16 2.20 2.10 1.89 1.66 1.45 1.19 0.87 0.63 0.45 0.27 0.16 0.10 0.06 0.04 0.02 0.02 0.01 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 4.02776 Average thickness (Biomass) (µm): 13.26090 Appended to file on: 2019-07-05 17:20:07 Image name: 24 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT 75% - C=0.ome.tif: PAO1 WT 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 Spots Measured (abs): 82088.00 916.00 2095.00 4276.00 8436.00 15009.00 23563.00 36550.00 51538.00 66500.00 76212.00 81229.00 80211.00 77352.00 71511.00 67513.00 59027.00 50872.00 41698.00 34214.00 27022.00 22994.00 18821.00 16245.00 13841.00 9667.00 5438.00 2450.00 916.00 285.00 79.00 7.00 1.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 7.83 0.09 0.20 0.41 0.80 1.43 2.25 3.49 4.92 6.34 7.27 7.75 7.65 7.38 6.82 6.44 5.63 4.85 3.98 3.26 2.58 2.19 1.79 1.55 1.32 0.92 0.52 0.23 0.09 0.03 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 12.40470 Average thickness (Biomass) (µm): 13.45829 Appended to file on: 2019-07-05 17:20:09 Image name: 25 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT 75% - C=1.ome.tif: PAO1 WT 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 Spots Measured (abs): 75806.00 2030.00 2588.00 3980.00 6281.00 9881.00 15493.00 23032.00 33720.00 47568.00 61634.00 74569.00 82222.00 89780.00 89233.00 87390.00 81006.00 72186.00 59608.00 44057.00 30507.00 19572.00 12476.00 8615.00 5914.00 3587.00 2385.00 1454.00 972.00 562.00 329.00 100.00 29.00 8.00 2.00 0.00 0.00 0.00 Spots Measured (%): 7.23 0.19 0.25 0.38 0.60 0.94 1.48 2.20 3.22 4.54 5.88 7.11 7.84 8.56 8.51 8.33 7.73 6.88 5.68 4.20 2.91 1.87 1.19 0.82 0.56 0.34 0.23 0.14 0.09 0.05 0.03 0.01 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 12.88401 Average thickness (Biomass) (µm): 13.88804 Appended to file on: 2019-07-05 17:20:12 Image name: 26 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 Inlet - C=0.ome.tif: PA3352 Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 Spots Measured (abs): 3195.00 11.00 22.00 70.00 178.00 306.00 534.00 1063.00 1894.00 3517.00 6236.00 9157.00 13147.00 19477.00 28097.00 36574.00 44053.00 50957.00 57593.00 51051.00 44526.00 45890.00 50882.00 49825.00 45325.00 45173.00 40379.00 30309.00 26170.00 26808.00 31423.00 40868.00 42522.00 41016.00 42826.00 44945.00 40115.00 25027.00 6462.00 842.00 101.00 10.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 0.30 0.00 0.00 0.01 0.02 0.03 0.05 0.10 0.18 0.34 0.59 0.87 1.25 1.86 2.68 3.49 4.20 4.86 5.49 4.87 4.25 4.38 4.85 4.75 4.32 4.31 3.85 2.89 2.50 2.56 3.00 3.90 4.06 3.91 4.08 4.29 3.83 2.39 0.62 0.08 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 24.28739 Average thickness (Biomass) (µm): 24.36162 Appended to file on: 2019-07-05 17:20:14 Image name: 27 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 Inlet - C=1.ome.tif: PA3352 Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 Spots Measured (abs): 22901.00 310.00 414.00 594.00 1012.00 1603.00 2785.00 4822.00 7684.00 11661.00 17077.00 24535.00 33285.00 43026.00 51694.00 58378.00 63699.00 66159.00 62118.00 57991.00 54244.00 49466.00 43101.00 38756.00 34060.00 31459.00 28193.00 26119.00 25020.00 25030.00 25813.00 25185.00 24718.00 24079.00 22549.00 19215.00 12239.00 5315.00 1717.00 431.00 106.00 13.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 2.18 0.03 0.04 0.06 0.10 0.15 0.27 0.46 0.73 1.11 1.63 2.34 3.17 4.10 4.93 5.57 6.07 6.31 5.92 5.53 5.17 4.72 4.11 3.70 3.25 3.00 2.69 2.49 2.39 2.39 2.46 2.40 2.36 2.30 2.15 1.83 1.17 0.51 0.16 0.04 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 20.33610 Average thickness (Biomass) (µm): 20.79016 Appended to file on: 2019-07-05 17:20:16 Image name: 28 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 25% - C=0.ome.tif: PA3352 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 516.00 11.00 29.00 81.00 137.00 282.00 471.00 839.00 1434.00 2566.00 4437.00 7473.00 11046.00 16506.00 24012.00 34252.00 49137.00 70744.00 93626.00 111709.00 111846.00 102456.00 84448.00 71225.00 63816.00 55349.00 50830.00 42359.00 26285.00 8965.00 1567.00 102.00 18.00 2.00 0.00 Spots Measured (%): 0.05 0.00 0.00 0.01 0.01 0.03 0.04 0.08 0.14 0.24 0.42 0.71 1.05 1.57 2.29 3.27 4.69 6.75 8.93 10.65 10.67 9.77 8.05 6.79 6.09 5.28 4.85 4.04 2.51 0.85 0.15 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 20.47654 Average thickness (Biomass) (µm): 20.48662 Appended to file on: 2019-07-05 17:20:19 Image name: 29 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 25% - C=1.ome.tif: PA3352 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 1681.00 116.00 142.00 257.00 458.00 770.00 1480.00 2696.00 4865.00 8466.00 14143.00 21508.00 31289.00 43077.00 56332.00 72419.00 89383.00 103128.00 105945.00 100165.00 85465.00 70325.00 59379.00 51823.00 45441.00 36879.00 23685.00 11203.00 4063.00 1261.00 454.00 190.00 59.00 29.00 0.00 Spots Measured (%): 0.16 0.01 0.01 0.02 0.04 0.07 0.14 0.26 0.46 0.81 1.35 2.05 2.98 4.11 5.37 6.91 8.52 9.84 10.10 9.55 8.15 6.71 5.66 4.94 4.33 3.52 2.26 1.07 0.39 0.12 0.04 0.02 0.01 0.00 0.00 Average thickness (Entire area) (µm): 18.22832 Average thickness (Biomass) (µm): 18.25759 Appended to file on: 2019-07-05 17:20:22 Image name: 3 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT Inlet - C=1.ome.tif: PAO1 WT Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 Spots Measured (abs): 34.00 0.00 0.00 1.00 1.00 1.00 6.00 4.00 16.00 33.00 127.00 359.00 1215.00 2991.00 5986.00 8920.00 12175.00 15196.00 18862.00 24628.00 34755.00 48724.00 64517.00 81294.00 92696.00 97903.00 99382.00 97699.00 87727.00 71876.00 54277.00 39195.00 28547.00 20753.00 14739.00 9872.00 6106.00 3514.00 1734.00 992.00 1719.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.12 0.29 0.57 0.85 1.16 1.45 1.80 2.35 3.31 4.65 6.15 7.75 8.84 9.34 9.48 9.32 8.37 6.85 5.18 3.74 2.72 1.98 1.41 0.94 0.58 0.34 0.17 0.09 0.16 Average thickness (Entire area) (µm): 25.54239 Average thickness (Biomass) (µm): 25.54322 Appended to file on: 2019-07-05 17:20:24 Image name: 30 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 50% - C=0.ome.tif: PA3352 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 3.00 8.00 22.00 82.00 129.00 311.00 580.00 979.00 1488.00 1904.00 2616.00 3834.00 5106.00 6826.00 8972.00 12085.00 15631.00 20695.00 27180.00 35197.00 43522.00 50891.00 54328.00 59684.00 68548.00 76172.00 86167.00 88807.00 82076.00 75128.00 61683.00 50176.00 34343.00 23282.00 18536.00 17468.00 9721.00 3465.00 877.00 53.00 0.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.01 0.03 0.06 0.09 0.14 0.18 0.25 0.37 0.49 0.65 0.86 1.15 1.49 1.97 2.59 3.36 4.15 4.85 5.18 5.69 6.54 7.26 8.22 8.47 7.83 7.16 5.88 4.79 3.28 2.22 1.77 1.67 0.93 0.33 0.08 0.01 0.00 0.00 Average thickness (Entire area) (µm): 34.28761 Average thickness (Biomass) (µm): 34.28761 Appended to file on: 2019-07-05 17:20:28 Image name: 31 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 50% - C=1.ome.tif: PA3352 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 Spots Measured (abs): 1.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 0.00 0.00 1.00 0.00 2.00 4.00 12.00 41.00 128.00 362.00 1039.00 2202.00 3949.00 5745.00 7844.00 11527.00 18230.00 26813.00 35727.00 46902.00 57275.00 64170.00 70402.00 74525.00 81859.00 87195.00 88622.00 83819.00 72203.00 59523.00 45942.00 31560.00 22716.00 17806.00 14379.00 9331.00 4125.00 1677.00 600.00 216.00 82.00 19.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.10 0.21 0.38 0.55 0.75 1.10 1.74 2.56 3.41 4.47 5.46 6.12 6.71 7.11 7.81 8.32 8.45 7.99 6.89 5.68 4.38 3.01 2.17 1.70 1.37 0.89 0.39 0.16 0.06 0.02 0.01 0.00 0.00 Average thickness (Entire area) (µm): 32.47474 Average thickness (Biomass) (µm): 32.47477 Appended to file on: 2019-07-05 17:20:30 Image name: 32 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 75% - C=0.ome.tif: PA3352 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 Spots Measured (abs): 117.00 34.00 42.00 95.00 196.00 333.00 557.00 869.00 1475.00 2340.00 3283.00 5041.00 7984.00 12136.00 16984.00 24231.00 33068.00 43448.00 54716.00 63490.00 67274.00 65348.00 62574.00 59251.00 49909.00 46879.00 49823.00 53226.00 48903.00 44821.00 42859.00 41462.00 37967.00 35437.00 28247.00 21368.00 13309.00 6995.00 2485.00 Spots Measured (%): 0.01 0.00 0.00 0.01 0.02 0.03 0.05 0.08 0.14 0.22 0.31 0.48 0.76 1.16 1.62 2.31 3.15 4.14 5.22 6.05 6.42 6.23 5.97 5.65 4.76 4.47 4.75 5.08 4.66 4.27 4.09 3.95 3.62 3.38 2.69 2.04 1.27 0.67 0.24 Average thickness (Entire area) (µm): 23.99851 Average thickness (Biomass) (µm): 24.00119 Appended to file on: 2019-07-05 17:20:32 Image name: 33 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 75% - C=1.ome.tif: PA3352 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 Spots Measured (abs): 241.00 45.00 50.00 102.00 198.00 398.00 697.00 1396.00 2597.00 5064.00 8568.00 13778.00 20560.00 28844.00 37442.00 46739.00 55900.00 62815.00 66194.00 68429.00 66278.00 59344.00 52460.00 48618.00 47202.00 48305.00 47186.00 44466.00 42130.00 40528.00 36717.00 30958.00 23553.00 17468.00 11544.00 7024.00 3500.00 991.00 247.00 Spots Measured (%): 0.02 0.00 0.00 0.01 0.02 0.04 0.07 0.13 0.25 0.48 0.82 1.31 1.96 2.75 3.57 4.46 5.33 5.99 6.31 6.53 6.32 5.66 5.00 4.64 4.50 4.61 4.50 4.24 4.02 3.87 3.50 2.95 2.25 1.67 1.10 0.67 0.33 0.09 0.02 Average thickness (Entire area) (µm): 21.72804 Average thickness (Biomass) (µm): 21.73303 Appended to file on: 2019-07-05 17:20:35 Image name: 34 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA3352 Outlet - C=0.ome.tif: PA3352 Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 Spots Measured (abs): 9.00 1.00 1.00 4.00 3.00 3.00 20.00 36.00 111.00 293.00 787.00 2016.00 3594.00 5461.00 7758.00 10785.00 16538.00 26733.00 38756.00 47217.00 50919.00 54623.00 54698.00 57179.00 56987.00 57208.00 56157.00 56490.00 53965.00 54601.00 48770.00 45319.00 42247.00 42049.00 39680.00 33413.00 24130.00 16831.00 11639.00 9296.00 7651.00 6347.00 3827.00 2387.00 2037.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.08 0.19 0.34 0.52 0.74 1.03 1.58 2.55 3.70 4.50 4.86 5.21 5.22 5.45 5.43 5.46 5.36 5.39 5.15 5.21 4.65 4.32 4.03 4.01 3.78 3.19 2.30 1.61 1.11 0.89 0.73 0.61 0.36 0.23 0.19 Average thickness (Entire area) (µm): 26.34735 Average thickness (Biomass) (µm): 26.34757 Appended to file on: 2019-07-05 17:20:37 Image name: 4 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT Outlet - C=0.ome.tif: PAO1 WT Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 61293.00 84.00 115.00 154.00 238.00 458.00 610.00 793.00 1184.00 1884.00 3353.00 5258.00 9456.00 16168.00 25981.00 38168.00 52481.00 67145.00 82863.00 94226.00 103700.00 105263.00 102732.00 92349.00 75074.00 52461.00 31401.00 15637.00 6530.00 1374.00 138.00 5.00 0.00 Spots Measured (%): 5.85 0.01 0.01 0.01 0.02 0.04 0.06 0.08 0.11 0.18 0.32 0.50 0.90 1.54 2.48 3.64 5.00 6.40 7.90 8.99 9.89 10.04 9.80 8.81 7.16 5.00 2.99 1.49 0.62 0.13 0.01 0.00 0.00 Average thickness (Entire area) (µm): 18.97384 Average thickness (Biomass) (µm): 20.15179 Appended to file on: 2019-07-05 17:20:39 Image name: 5 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT Outlet - C=1.ome.tif: PAO1 WT Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 49970.00 214.00 210.00 318.00 472.00 764.00 1301.00 2041.00 3260.00 5045.00 8079.00 13067.00 21296.00 33951.00 51001.00 69185.00 86723.00 100850.00 110005.00 111546.00 107009.00 90536.00 70301.00 48102.00 29733.00 17153.00 9519.00 4021.00 1757.00 664.00 302.00 144.00 37.00 Spots Measured (%): 4.77 0.02 0.02 0.03 0.05 0.07 0.12 0.19 0.31 0.48 0.77 1.25 2.03 3.24 4.86 6.60 8.27 9.62 10.49 10.64 10.21 8.63 6.70 4.59 2.84 1.64 0.91 0.38 0.17 0.06 0.03 0.01 0.00 Average thickness (Entire area) (µm): 17.40089 Average thickness (Biomass) (µm): 18.27162 Appended to file on: 2019-07-05 17:20:41 Image name: 6 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT 25% - C=0.ome.tif: PAO1 WT 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 Spots Measured (abs): 4474.00 16.00 40.00 95.00 231.00 603.00 1674.00 4182.00 9237.00 16437.00 24275.00 32115.00 39344.00 45130.00 47157.00 47448.00 44027.00 40659.00 35928.00 33080.00 31301.00 31392.00 33678.00 36946.00 37770.00 39604.00 41556.00 43760.00 43617.00 44785.00 40232.00 33359.00 26996.00 22669.00 19908.00 19900.00 20216.00 18991.00 15567.00 10465.00 5982.00 3730.00 Spots Measured (%): 0.43 0.00 0.00 0.01 0.02 0.06 0.16 0.40 0.88 1.57 2.32 3.06 3.75 4.30 4.50 4.52 4.20 3.88 3.43 3.15 2.99 2.99 3.21 3.52 3.60 3.78 3.96 4.17 4.16 4.27 3.84 3.18 2.57 2.16 1.90 1.90 1.93 1.81 1.48 1.00 0.57 0.36 Average thickness (Entire area) (µm): 22.46787 Average thickness (Biomass) (µm): 22.56415 Appended to file on: 2019-07-05 17:20:43 Image name: 7 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PAO1 WT 25% - C=1.ome.tif: PAO1 WT 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 Spots Measured (abs): 500909.00 3153.00 4075.00 5903.00 9010.00 12851.00 17865.00 24132.00 29869.00 34879.00 38329.00 40825.00 42124.00 42080.00 39703.00 36120.00 30963.00 25684.00 20918.00 17642.00 14538.00 11479.00 9253.00 7231.00 5103.00 4072.00 3165.00 2666.00 2394.00 2041.00 1641.00 1420.00 1256.00 1131.00 988.00 861.00 886.00 701.00 370.00 216.00 101.00 29.00 Spots Measured (%): 47.77 0.30 0.39 0.56 0.86 1.23 1.70 2.30 2.85 3.33 3.66 3.89 4.02 4.01 3.79 3.44 2.95 2.45 1.99 1.68 1.39 1.09 0.88 0.69 0.49 0.39 0.30 0.25 0.23 0.19 0.16 0.14 0.12 0.11 0.09 0.08 0.08 0.07 0.04 0.02 0.01 0.00 Average thickness (Entire area) (µm): 6.97030 Average thickness (Biomass) (µm): 13.34549 Appended to file on: 2019-07-05 17:20:45 Image name: 8 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 50% - C=0.ome.tif: PA0996 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 Spots Measured (abs): 66725.00 10677.00 15847.00 21738.00 27711.00 30337.00 28523.00 22708.00 17571.00 13494.00 11295.00 9177.00 8525.00 8630.00 8856.00 9293.00 9877.00 10930.00 12313.00 13955.00 16267.00 19355.00 26977.00 41838.00 64939.00 86008.00 99102.00 92434.00 85023.00 68450.00 47601.00 26633.00 10600.00 3813.00 1141.00 213.00 Spots Measured (%): 6.36 1.02 1.51 2.07 2.64 2.89 2.72 2.17 1.68 1.29 1.08 0.88 0.81 0.82 0.84 0.89 0.94 1.04 1.17 1.33 1.55 1.85 2.57 3.99 6.19 8.20 9.45 8.82 8.11 6.53 4.54 2.54 1.01 0.36 0.11 0.02 Average thickness (Entire area) (µm): 19.71979 Average thickness (Biomass) (µm): 21.05991 Appended to file on: 2019-07-05 17:20:47 Image name: 9 - Ray Allan 72h PAO1 + mutants microfluidics 19.04.19.lif - PA0996 50% - C=1.ome.tif: PA0996 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 Spots Measured (abs): 182814.00 8343.00 10636.00 15027.00 21704.00 28881.00 35398.00 40109.00 43541.00 45008.00 43185.00 40127.00 36294.00 33670.00 30776.00 28970.00 28425.00 29312.00 32038.00 35881.00 39478.00 41956.00 42634.00 39368.00 33628.00 26480.00 19570.00 12828.00 8791.00 5695.00 3280.00 1984.00 1237.00 646.00 402.00 460.00 Spots Measured (%): 17.43 0.80 1.01 1.43 2.07 2.75 3.38 3.83 4.15 4.29 4.12 3.83 3.46 3.21 2.94 2.76 2.71 2.80 3.06 3.42 3.76 4.00 4.07 3.75 3.21 2.53 1.87 1.22 0.84 0.54 0.31 0.19 0.12 0.06 0.04 0.04 Average thickness (Entire area) (µm): 12.27589 Average thickness (Biomass) (µm): 14.86806