Thickness distribution File created on: 2019-07-06 10:36:03 Image name: 1 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - Inlet - C=0.ome.tif: A - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 1680.00 4.00 2.00 8.00 22.00 105.00 315.00 777.00 1763.00 3746.00 6845.00 11743.00 19721.00 31158.00 42009.00 57686.00 74618.00 98270.00 118942.00 132818.00 129046.00 113808.00 87566.00 54162.00 30829.00 17215.00 8731.00 3459.00 1220.00 231.00 48.00 29.00 0.00 0.00 0.00 Spots Measured (%): 0.16 0.00 0.00 0.00 0.00 0.01 0.03 0.07 0.17 0.36 0.65 1.12 1.88 2.97 4.01 5.50 7.12 9.37 11.34 12.67 12.31 10.85 8.35 5.17 2.94 1.64 0.83 0.33 0.12 0.02 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 18.64298 Average thickness (Biomass) (µm): 18.67290 Appended to file on: 2019-07-06 10:36:07 Image name: 10 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - Outlet - C=1.ome.tif: A - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 51.98 52.98 53.98 54.98 55.98 56.98 Spots Measured (abs): 6507.00 146.00 58.00 59.00 44.00 44.00 38.00 33.00 45.00 47.00 36.00 44.00 43.00 71.00 61.00 51.00 84.00 93.00 89.00 132.00 129.00 141.00 155.00 220.00 235.00 374.00 596.00 876.00 1452.00 2466.00 4192.00 6775.00 10602.00 15837.00 21828.00 27681.00 33696.00 39366.00 45413.00 54676.00 63388.00 68735.00 71717.00 71436.00 66785.00 62986.00 58740.00 55280.00 47194.00 40205.00 32714.00 27070.00 23035.00 20616.00 17760.00 16052.00 14081.00 16347.00 Spots Measured (%): 0.62 0.01 0.01 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.01 0.00 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.02 0.02 0.04 0.06 0.08 0.14 0.24 0.40 0.65 1.01 1.51 2.08 2.64 3.21 3.75 4.33 5.21 6.05 6.56 6.84 6.81 6.37 6.01 5.60 5.27 4.50 3.83 3.12 2.58 2.20 1.97 1.69 1.53 1.34 1.56 Average thickness (Entire area) (µm): 43.13565 Average thickness (Biomass) (µm): 43.40500 Appended to file on: 2019-07-06 10:36:09 Image name: 11 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - 50% - C=0.ome.tif: B - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 Spots Measured (abs): 8399.00 1689.00 3524.00 6780.00 13424.00 23764.00 40232.00 57413.00 70059.00 75939.00 79150.00 78767.00 66627.00 55701.00 47967.00 44793.00 46945.00 49577.00 51248.00 48467.00 44746.00 35575.00 28969.00 23087.00 18092.00 12735.00 8000.00 4456.00 2451.00 Spots Measured (%): 0.80 0.16 0.34 0.65 1.28 2.27 3.84 5.48 6.68 7.24 7.55 7.51 6.35 5.31 4.57 4.27 4.48 4.73 4.89 4.62 4.27 3.39 2.76 2.20 1.73 1.21 0.76 0.42 0.23 Average thickness (Entire area) (µm): 13.35784 Average thickness (Biomass) (µm): 13.46569 Appended to file on: 2019-07-06 10:36:12 Image name: 12 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - 50% - C=1.ome.tif: B - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 Spots Measured (abs): 271741.00 7016.00 8005.00 13125.00 21093.00 30794.00 42903.00 54618.00 63887.00 67858.00 72215.00 74496.00 67529.00 59684.00 49045.00 37992.00 30080.00 23206.00 17138.00 12181.00 8285.00 5388.00 3566.00 2283.00 1487.00 1061.00 768.00 572.00 560.00 Spots Measured (%): 25.92 0.67 0.76 1.25 2.01 2.94 4.09 5.21 6.09 6.47 6.89 7.10 6.44 5.69 4.68 3.62 2.87 2.21 1.63 1.16 0.79 0.51 0.34 0.22 0.14 0.10 0.07 0.05 0.05 Average thickness (Entire area) (µm): 7.99462 Average thickness (Biomass) (µm): 10.79118 Appended to file on: 2019-07-06 10:36:14 Image name: 13 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - 75% - C=0.ome.tif: B - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 Spots Measured (abs): 0.00 0.00 0.00 0.00 1.00 3.00 8.00 40.00 236.00 900.00 3255.00 10404.00 27183.00 57341.00 104711.00 159551.00 184230.00 167215.00 132026.00 84790.00 52188.00 30198.00 17127.00 9787.00 7382.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.09 0.31 0.99 2.59 5.47 9.99 15.22 17.57 15.95 12.59 8.09 4.98 2.88 1.63 0.93 0.70 Average thickness (Entire area) (µm): 16.51552 Average thickness (Biomass) (µm): 16.51552 Appended to file on: 2019-07-06 10:36:16 Image name: 14 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - 75% - C=1.ome.tif: B - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 Spots Measured (abs): 2678.00 63.00 46.00 90.00 186.00 329.00 598.00 1143.00 2166.00 4268.00 8117.00 15325.00 28055.00 49828.00 84700.00 125773.00 151038.00 159179.00 147983.00 111545.00 75356.00 42995.00 22073.00 10110.00 4932.00 Spots Measured (%): 0.26 0.01 0.00 0.01 0.02 0.03 0.06 0.11 0.21 0.41 0.77 1.46 2.68 4.75 8.08 11.99 14.40 15.18 14.11 10.64 7.19 4.10 2.11 0.96 0.47 Average thickness (Entire area) (µm): 16.71187 Average thickness (Biomass) (µm): 16.75466 Appended to file on: 2019-07-06 10:36:17 Image name: 15 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - Outlet - C=0.ome.tif: B - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 214.00 29.00 59.00 181.00 449.00 1216.00 3080.00 7344.00 15450.00 28136.00 43005.00 60233.00 76732.00 97326.00 115225.00 122194.00 112775.00 90918.00 72426.00 57838.00 46037.00 36625.00 27160.00 17186.00 9217.00 4700.00 2821.00 Spots Measured (%): 0.02 0.00 0.01 0.02 0.04 0.12 0.29 0.70 1.47 2.68 4.10 5.74 7.32 9.28 10.99 11.65 10.76 8.67 6.91 5.52 4.39 3.49 2.59 1.64 0.88 0.45 0.27 Average thickness (Entire area) (µm): 15.28023 Average thickness (Biomass) (µm): 15.28334 Appended to file on: 2019-07-06 10:36:19 Image name: 16 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - Outlet - C=1.ome.tif: B - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 44719.00 1144.00 1033.00 1327.00 2051.00 3231.00 5371.00 8627.00 14053.00 21532.00 30738.00 42477.00 55672.00 72488.00 89082.00 100988.00 102215.00 92518.00 80659.00 67505.00 55947.00 48384.00 39839.00 29462.00 18144.00 11169.00 8201.00 Spots Measured (%): 4.26 0.11 0.10 0.13 0.20 0.31 0.51 0.82 1.34 2.05 2.93 4.05 5.31 6.91 8.50 9.63 9.75 8.82 7.69 6.44 5.34 4.61 3.80 2.81 1.73 1.07 0.78 Average thickness (Entire area) (µm): 15.38490 Average thickness (Biomass) (µm): 16.07025 Appended to file on: 2019-07-06 10:36:21 Image name: 17 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - Inlet - C=0.ome.tif: C - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 12735.00 949.00 1202.00 2129.00 3473.00 5775.00 10052.00 15314.00 23049.00 32197.00 44119.00 56701.00 68549.00 81457.00 89245.00 98019.00 106321.00 104119.00 92170.00 72711.00 52661.00 36431.00 21523.00 10308.00 4478.00 1845.00 1044.00 Spots Measured (%): 1.21 0.09 0.11 0.20 0.33 0.55 0.96 1.46 2.20 3.07 4.21 5.41 6.54 7.77 8.51 9.35 10.14 9.93 8.79 6.93 5.02 3.47 2.05 0.98 0.43 0.18 0.10 Average thickness (Entire area) (µm): 14.85537 Average thickness (Biomass) (µm): 15.03801 Appended to file on: 2019-07-06 10:36:23 Image name: 18 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - Inlet - C=1.ome.tif: C - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 Spots Measured (abs): 358772.00 4962.00 4862.00 6707.00 9382.00 12986.00 17088.00 22387.00 29578.00 37474.00 43736.00 49953.00 54214.00 56883.00 57568.00 56640.00 55037.00 49467.00 38963.00 28130.00 19755.00 14352.00 9221.00 5065.00 2623.00 1492.00 1279.00 Spots Measured (%): 34.22 0.47 0.46 0.64 0.89 1.24 1.63 2.13 2.82 3.57 4.17 4.76 5.17 5.42 5.49 5.40 5.25 4.72 3.72 2.68 1.88 1.37 0.88 0.48 0.25 0.14 0.12 Average thickness (Entire area) (µm): 8.68491 Average thickness (Biomass) (µm): 13.20200 Appended to file on: 2019-07-06 10:36:25 Image name: 19 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - 25% - C=0.ome.tif: C - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 Spots Measured (abs): 1890.00 326.00 739.00 1616.00 3713.00 7778.00 15003.00 25801.00 39408.00 53651.00 64134.00 70705.00 67009.00 59631.00 50048.00 39626.00 31184.00 25397.00 24542.00 25176.00 27261.00 31065.00 36009.00 39476.00 42590.00 46676.00 46793.00 46892.00 43812.00 36856.00 24377.00 13276.00 4968.00 1148.00 Spots Measured (%): 0.18 0.03 0.07 0.15 0.35 0.74 1.43 2.46 3.76 5.12 6.12 6.74 6.39 5.69 4.77 3.78 2.97 2.42 2.34 2.40 2.60 2.96 3.43 3.76 4.06 4.45 4.46 4.47 4.18 3.51 2.32 1.27 0.47 0.11 Average thickness (Entire area) (µm): 17.61688 Average thickness (Biomass) (µm): 17.64869 Appended to file on: 2019-07-06 10:36:27 Image name: 2 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - Inlet - C=1.ome.tif: A - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 12042.00 215.00 136.00 224.00 442.00 730.00 1292.00 2202.00 3861.00 6482.00 10328.00 16734.00 25872.00 38255.00 51151.00 65620.00 79669.00 97005.00 112919.00 124073.00 118991.00 100713.00 77161.00 49146.00 28334.00 14515.00 6468.00 2554.00 1023.00 311.00 59.00 29.00 18.00 2.00 0.00 Spots Measured (%): 1.15 0.02 0.01 0.02 0.04 0.07 0.12 0.21 0.37 0.62 0.98 1.60 2.47 3.65 4.88 6.26 7.60 9.25 10.77 11.83 11.35 9.60 7.36 4.69 2.70 1.38 0.62 0.24 0.10 0.03 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 17.99226 Average thickness (Biomass) (µm): 18.20129 Appended to file on: 2019-07-06 10:36:28 Image name: 20 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - 25% - C=1.ome.tif: C - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 Spots Measured (abs): 542094.00 4156.00 4135.00 6495.00 10060.00 15502.00 21712.00 28897.00 36647.00 43534.00 49687.00 54529.00 53843.00 49841.00 40915.00 29321.00 19828.00 12723.00 8205.00 5669.00 3982.00 2450.00 1653.00 953.00 575.00 407.00 249.00 197.00 148.00 95.00 37.00 12.00 11.00 14.00 Spots Measured (%): 51.70 0.40 0.39 0.62 0.96 1.48 2.07 2.76 3.49 4.15 4.74 5.20 5.13 4.75 3.90 2.80 1.89 1.21 0.78 0.54 0.38 0.23 0.16 0.09 0.05 0.04 0.02 0.02 0.01 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 5.33310 Average thickness (Biomass) (µm): 11.04118 Appended to file on: 2019-07-06 10:36:30 Image name: 21 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - 50% - C=0.ome.tif: C - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 3555.00 273.00 362.00 572.00 1051.00 1930.00 3544.00 6711.00 13478.00 23795.00 39509.00 58851.00 82670.00 98673.00 105009.00 101653.00 89067.00 75288.00 60123.00 50795.00 47319.00 40008.00 33804.00 28773.00 22700.00 17955.00 13612.00 9449.00 7173.00 5101.00 3435.00 2338.00 Spots Measured (%): 0.34 0.03 0.03 0.05 0.10 0.18 0.34 0.64 1.29 2.27 3.77 5.61 7.88 9.41 10.01 9.69 8.49 7.18 5.73 4.84 4.51 3.82 3.22 2.74 2.16 1.71 1.30 0.90 0.68 0.49 0.33 0.22 Average thickness (Entire area) (µm): 16.00764 Average thickness (Biomass) (µm): 16.06209 Appended to file on: 2019-07-06 10:36:32 Image name: 22 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - 50% - C=1.ome.tif: C - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 300605.00 4900.00 4155.00 5188.00 7655.00 10909.00 15870.00 22634.00 31180.00 41054.00 51026.00 62164.00 73174.00 77338.00 74852.00 66954.00 52966.00 38240.00 26405.00 19151.00 15736.00 11519.00 8519.00 7298.00 4968.00 3507.00 2739.00 2151.00 1772.00 1432.00 1108.00 1407.00 Spots Measured (%): 28.67 0.47 0.40 0.49 0.73 1.04 1.51 2.16 2.97 3.92 4.87 5.93 6.98 7.38 7.14 6.39 5.05 3.65 2.52 1.83 1.50 1.10 0.81 0.70 0.47 0.33 0.26 0.21 0.17 0.14 0.11 0.13 Average thickness (Entire area) (µm): 9.40908 Average thickness (Biomass) (µm): 13.19053 Appended to file on: 2019-07-06 10:36:34 Image name: 23 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - 75% - C=0.ome.tif: C - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 2.00 0.00 1.00 0.00 1.00 2.00 2.00 11.00 22.00 86.00 269.00 873.00 2443.00 6254.00 12729.00 22286.00 35468.00 50688.00 69471.00 87080.00 102092.00 114695.00 111545.00 98729.00 84851.00 66965.00 54477.00 42996.00 33523.00 23670.00 14171.00 7795.00 5379.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.08 0.23 0.60 1.21 2.13 3.38 4.83 6.63 8.30 9.74 10.94 10.64 9.42 8.09 6.39 5.20 4.10 3.20 2.26 1.35 0.74 0.51 Average thickness (Entire area) (µm): 21.83036 Average thickness (Biomass) (µm): 21.83040 Appended to file on: 2019-07-06 10:36:37 Image name: 24 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - 75% - C=1.ome.tif: C - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 Spots Measured (abs): 1980.00 72.00 48.00 62.00 60.00 136.00 210.00 403.00 592.00 933.00 1586.00 2834.00 4709.00 8402.00 13548.00 20193.00 28687.00 39120.00 51672.00 65439.00 79327.00 93223.00 98770.00 95928.00 91055.00 78800.00 68294.00 59119.00 48538.00 36332.00 24884.00 16707.00 16913.00 Spots Measured (%): 0.19 0.01 0.00 0.01 0.01 0.01 0.02 0.04 0.06 0.09 0.15 0.27 0.45 0.80 1.29 1.93 2.74 3.73 4.93 6.24 7.57 8.89 9.42 9.15 8.68 7.51 6.51 5.64 4.63 3.46 2.37 1.59 1.61 Average thickness (Entire area) (µm): 22.58607 Average thickness (Biomass) (µm): 22.62880 Appended to file on: 2019-07-06 10:36:40 Image name: 25 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - Outlet - C=0.ome.tif: C - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 Spots Measured (abs): 2747.00 190.00 177.00 184.00 253.00 369.00 632.00 1097.00 2056.00 3792.00 6632.00 10866.00 16465.00 22252.00 27214.00 32272.00 38024.00 45292.00 53866.00 62307.00 68886.00 70271.00 70807.00 66191.00 64037.00 60232.00 55576.00 51211.00 44290.00 38272.00 32300.00 25796.00 20699.00 15139.00 11335.00 8531.00 6420.00 4822.00 3494.00 2209.00 1371.00 Spots Measured (%): 0.26 0.02 0.02 0.02 0.02 0.04 0.06 0.10 0.20 0.36 0.63 1.04 1.57 2.12 2.60 3.08 3.63 4.32 5.14 5.94 6.57 6.70 6.75 6.31 6.11 5.74 5.30 4.88 4.22 3.65 3.08 2.46 1.97 1.44 1.08 0.81 0.61 0.46 0.33 0.21 0.13 Average thickness (Entire area) (µm): 22.48793 Average thickness (Biomass) (µm): 22.54699 Appended to file on: 2019-07-06 10:36:42 Image name: 26 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - C - Outlet - C=1.ome.tif: C - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 Spots Measured (abs): 52449.00 1073.00 857.00 1028.00 1307.00 1598.00 2131.00 3156.00 4426.00 6153.00 8856.00 12558.00 16324.00 21502.00 25311.00 29142.00 34926.00 42146.00 50883.00 58642.00 64226.00 64309.00 64512.00 60481.00 58958.00 54557.00 50939.00 47469.00 42033.00 35555.00 30046.00 24040.00 19814.00 15028.00 11439.00 9363.00 7783.00 5523.00 3687.00 2241.00 2105.00 Spots Measured (%): 5.00 0.10 0.08 0.10 0.12 0.15 0.20 0.30 0.42 0.59 0.84 1.20 1.56 2.05 2.41 2.78 3.33 4.02 4.85 5.59 6.13 6.13 6.15 5.77 5.62 5.20 4.86 4.53 4.01 3.39 2.87 2.29 1.89 1.43 1.09 0.89 0.74 0.53 0.35 0.21 0.20 Average thickness (Entire area) (µm): 21.23344 Average thickness (Biomass) (µm): 22.35144 Appended to file on: 2019-07-06 10:36:46 Image name: 27 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - 25% - C=0.ome.tif: B - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 51.98 52.98 53.98 54.98 55.98 56.98 57.98 58.98 59.98 60.98 61.98 62.98 63.98 64.98 65.98 66.98 67.98 68.98 69.98 70.98 71.98 72.98 73.98 74.98 75.98 76.98 77.98 78.98 79.98 80.98 81.98 82.98 83.97 84.97 85.97 86.97 87.97 Spots Measured (abs): 38.00 0.00 0.00 0.00 1.00 0.00 0.00 7.00 8.00 15.00 26.00 33.00 74.00 123.00 204.00 296.00 464.00 590.00 869.00 1139.00 1594.00 2095.00 2719.00 3377.00 4301.00 5180.00 6539.00 7274.00 8089.00 8979.00 8916.00 9174.00 9583.00 10876.00 11508.00 13424.00 15691.00 16896.00 17934.00 19305.00 21013.00 22949.00 23911.00 25295.00 24130.00 24300.00 24081.00 24130.00 23944.00 25104.00 24831.00 24739.00 24517.00 23757.00 24700.00 24497.00 23960.00 25130.00 23903.00 23762.00 22960.00 21550.00 20541.00 19541.00 19049.00 19194.00 19435.00 19345.00 18717.00 18145.00 16978.00 15681.00 13607.00 12835.00 11722.00 10594.00 9931.00 8499.00 7789.00 6937.00 6449.00 6532.00 6047.00 5917.00 4868.00 4711.00 4732.00 6630.00 19646.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.01 0.02 0.03 0.04 0.06 0.08 0.11 0.15 0.20 0.26 0.32 0.41 0.49 0.62 0.69 0.77 0.86 0.85 0.87 0.91 1.04 1.10 1.28 1.50 1.61 1.71 1.84 2.00 2.19 2.28 2.41 2.30 2.32 2.30 2.30 2.28 2.39 2.37 2.36 2.34 2.27 2.36 2.34 2.29 2.40 2.28 2.27 2.19 2.06 1.96 1.86 1.82 1.83 1.85 1.84 1.78 1.73 1.62 1.50 1.30 1.22 1.12 1.01 0.95 0.81 0.74 0.66 0.62 0.62 0.58 0.56 0.46 0.45 0.45 0.63 1.87 Average thickness (Entire area) (µm): 54.11879 Average thickness (Biomass) (µm): 54.12076 Appended to file on: 2019-07-06 10:36:51 Image name: 28 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - B - 25% - C=1.ome.tif: B - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 51.98 52.98 53.98 54.98 55.98 56.98 57.98 58.98 59.98 60.98 61.98 62.98 63.98 64.98 65.98 66.98 67.98 68.98 69.98 70.98 71.98 72.98 73.98 74.98 75.98 76.98 77.98 78.98 79.98 80.98 81.98 82.98 83.97 84.97 85.97 86.97 87.97 Spots Measured (abs): 7974.00 56.00 21.00 14.00 19.00 29.00 33.00 43.00 59.00 51.00 86.00 117.00 131.00 229.00 248.00 359.00 427.00 517.00 705.00 878.00 1195.00 1462.00 1857.00 2387.00 2886.00 3507.00 4416.00 5060.00 5883.00 6731.00 7325.00 8155.00 8982.00 10714.00 11131.00 12778.00 14165.00 15776.00 16834.00 18523.00 18925.00 20554.00 21233.00 22875.00 22511.00 23219.00 23106.00 23859.00 25016.00 25383.00 26257.00 26645.00 27063.00 27223.00 27525.00 27691.00 27459.00 28538.00 27218.00 26414.00 25103.00 23514.00 22291.00 20998.00 20655.00 19791.00 19577.00 19301.00 17835.00 17001.00 15985.00 14729.00 13800.00 12255.00 11564.00 10878.00 9773.00 8658.00 7882.00 7576.00 6707.00 6556.00 6117.00 5878.00 5194.00 5052.00 4868.00 6001.00 12560.00 Spots Measured (%): 0.76 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.00 0.01 0.01 0.01 0.02 0.02 0.03 0.04 0.05 0.07 0.08 0.11 0.14 0.18 0.23 0.28 0.33 0.42 0.48 0.56 0.64 0.70 0.78 0.86 1.02 1.06 1.22 1.35 1.50 1.61 1.77 1.80 1.96 2.02 2.18 2.15 2.21 2.20 2.28 2.39 2.42 2.50 2.54 2.58 2.60 2.62 2.64 2.62 2.72 2.60 2.52 2.39 2.24 2.13 2.00 1.97 1.89 1.87 1.84 1.70 1.62 1.52 1.40 1.32 1.17 1.10 1.04 0.93 0.83 0.75 0.72 0.64 0.63 0.58 0.56 0.50 0.48 0.46 0.57 1.20 Average thickness (Entire area) (µm): 54.23706 Average thickness (Biomass) (µm): 54.65267 Appended to file on: 2019-07-06 10:36:53 Image name: 3 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - 25% - C=0.ome.tif: A - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 1.00 0.00 3.00 11.00 22.00 90.00 275.00 1071.00 2983.00 5889.00 10581.00 13803.00 18250.00 22570.00 26105.00 29432.00 32066.00 36163.00 40986.00 47117.00 63102.00 80276.00 101855.00 129509.00 140531.00 113726.00 74403.00 36994.00 16383.00 3745.00 578.00 52.00 4.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.10 0.28 0.56 1.01 1.32 1.74 2.15 2.49 2.81 3.06 3.45 3.91 4.49 6.02 7.66 9.71 12.35 13.40 10.85 7.10 3.53 1.56 0.36 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 27.28484 Average thickness (Biomass) (µm): 27.28484 Appended to file on: 2019-07-06 10:36:55 Image name: 4 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - 25% - C=1.ome.tif: A - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 Spots Measured (abs): 374458.00 6208.00 4738.00 4904.00 5696.00 6681.00 8119.00 10048.00 12183.00 15488.00 19203.00 24958.00 32936.00 41895.00 49079.00 51783.00 52683.00 49515.00 45192.00 41414.00 35589.00 29809.00 24796.00 20497.00 17573.00 14971.00 13237.00 10605.00 8276.00 6133.00 4234.00 2481.00 1423.00 812.00 466.00 196.00 138.00 87.00 58.00 13.00 1.00 0.00 0.00 0.00 Spots Measured (%): 35.71 0.59 0.45 0.47 0.54 0.64 0.77 0.96 1.16 1.48 1.83 2.38 3.14 4.00 4.68 4.94 5.02 4.72 4.31 3.95 3.39 2.84 2.36 1.95 1.68 1.43 1.26 1.01 0.79 0.58 0.40 0.24 0.14 0.08 0.04 0.02 0.01 0.01 0.01 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 10.58119 Average thickness (Biomass) (µm): 16.45881 Appended to file on: 2019-07-06 10:36:57 Image name: 5 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - 50% - C=0.ome.tif: A - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 52602.00 1865.00 2906.00 5191.00 10016.00 17782.00 27304.00 35777.00 42892.00 48229.00 50993.00 53754.00 49572.00 43231.00 37896.00 35866.00 38087.00 41950.00 44571.00 49468.00 49723.00 46001.00 47357.00 43891.00 40544.00 36653.00 32906.00 30207.00 18800.00 8474.00 2941.00 1127.00 Spots Measured (%): 5.02 0.18 0.28 0.50 0.96 1.70 2.60 3.41 4.09 4.60 4.86 5.13 4.73 4.12 3.61 3.42 3.63 4.00 4.25 4.72 4.74 4.39 4.52 4.19 3.87 3.50 3.14 2.88 1.79 0.81 0.28 0.11 Average thickness (Entire area) (µm): 15.44745 Average thickness (Biomass) (µm): 16.26330 Appended to file on: 2019-07-06 10:36:59 Image name: 6 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - 50% - C=1.ome.tif: A - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 488945.00 10903.00 10755.00 13873.00 18799.00 25228.00 31874.00 38683.00 45834.00 52773.00 57373.00 59630.00 53717.00 43150.00 31835.00 22035.00 16145.00 10660.00 6317.00 3849.00 2289.00 1369.00 878.00 583.00 353.00 252.00 192.00 142.00 88.00 26.00 19.00 7.00 Spots Measured (%): 46.63 1.04 1.03 1.32 1.79 2.41 3.04 3.69 4.37 5.03 5.47 5.69 5.12 4.12 3.04 2.10 1.54 1.02 0.60 0.37 0.22 0.13 0.08 0.06 0.03 0.02 0.02 0.01 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 5.29291 Average thickness (Biomass) (µm): 9.91728 Appended to file on: 2019-07-06 10:37:01 Image name: 7 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - 75% - C=0.ome.tif: A - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 Spots Measured (abs): 95.00 31.00 28.00 78.00 149.00 356.00 1031.00 2467.00 5645.00 11556.00 19713.00 30354.00 42627.00 57729.00 71222.00 82864.00 87320.00 88642.00 92025.00 91843.00 84400.00 76042.00 61545.00 45617.00 32547.00 22585.00 16386.00 11290.00 6819.00 3781.00 1789.00 Spots Measured (%): 0.01 0.00 0.00 0.01 0.01 0.03 0.10 0.24 0.54 1.10 1.88 2.89 4.07 5.51 6.79 7.90 8.33 8.45 8.78 8.76 8.05 7.25 5.87 4.35 3.10 2.15 1.56 1.08 0.65 0.36 0.17 Average thickness (Entire area) (µm): 17.76433 Average thickness (Biomass) (µm): 17.76594 Appended to file on: 2019-07-06 10:37:03 Image name: 8 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - 75% - C=1.ome.tif: A - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 Spots Measured (abs): 14272.00 611.00 521.00 680.00 1095.00 1730.00 2928.00 4770.00 7446.00 11514.00 16518.00 23587.00 31695.00 42832.00 55085.00 68096.00 75970.00 80174.00 85949.00 88697.00 87273.00 82613.00 71581.00 57435.00 43709.00 30378.00 21395.00 16336.00 10936.00 6922.00 5828.00 Spots Measured (%): 1.36 0.06 0.05 0.06 0.10 0.16 0.28 0.45 0.71 1.10 1.58 2.25 3.02 4.08 5.25 6.49 7.25 7.65 8.20 8.46 8.32 7.88 6.83 5.48 4.17 2.90 2.04 1.56 1.04 0.66 0.56 Average thickness (Entire area) (µm): 18.17140 Average thickness (Biomass) (µm): 18.42214 Appended to file on: 2019-07-06 10:37:07 Image name: 9 - Ray Allan 72h PAO1 WT microfluidics 24.05.19.lif - A - Outlet - C=0.ome.tif: A - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 42.99 43.99 44.99 45.99 46.99 47.99 48.99 49.99 50.98 51.98 52.98 53.98 54.98 55.98 56.98 Spots Measured (abs): 193.00 11.00 4.00 6.00 1.00 1.00 0.00 0.00 0.00 1.00 0.00 0.00 1.00 0.00 0.00 0.00 2.00 1.00 4.00 5.00 3.00 5.00 12.00 19.00 35.00 54.00 107.00 254.00 521.00 1405.00 3232.00 6915.00 12245.00 19873.00 27643.00 35542.00 42718.00 49837.00 55937.00 66028.00 72759.00 75011.00 74559.00 70838.00 64798.00 59496.00 53681.00 47919.00 39220.00 33580.00 27880.00 23416.00 20100.00 17509.00 14769.00 11985.00 9862.00 8579.00 Spots Measured (%): 0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.01 0.02 0.05 0.13 0.31 0.66 1.17 1.90 2.64 3.39 4.07 4.75 5.33 6.30 6.94 7.15 7.11 6.76 6.18 5.67 5.12 4.57 3.74 3.20 2.66 2.23 1.92 1.67 1.41 1.14 0.94 0.82 Average thickness (Entire area) (µm): 42.61098 Average thickness (Biomass) (µm): 42.61882