Thickness distribution File created on: 2019-07-05 16:47:30 Image name: 1 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - Inlet - C=0.ome.tif: A - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 15525.00 21.00 131.00 874.00 3727.00 11556.00 24275.00 36387.00 51024.00 70417.00 58938.00 52055.00 48743.00 45798.00 42787.00 42251.00 42019.00 43299.00 41663.00 38634.00 34575.00 31439.00 28684.00 27335.00 27314.00 31834.00 36501.00 41295.00 38239.00 28402.00 19170.00 11929.00 8554.00 7219.00 5962.00 Spots Measured (%): 1.48 0.00 0.01 0.08 0.36 1.10 2.32 3.47 4.87 6.72 5.62 4.96 4.65 4.37 4.08 4.03 4.01 4.13 3.97 3.68 3.30 3.00 2.74 2.61 2.60 3.04 3.48 3.94 3.65 2.71 1.83 1.14 0.82 0.69 0.57 Average thickness (Entire area) (µm): 16.84264 Average thickness (Biomass) (µm): 17.09575 Appended to file on: 2019-07-05 16:47:33 Image name: 10 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - Outlet - C=1.ome.tif: A - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 Spots Measured (abs): 6242.00 232.00 340.00 700.00 1544.00 2872.00 4960.00 8367.00 13661.00 21043.00 29834.00 39041.00 49160.00 57685.00 66257.00 75467.00 84532.00 95599.00 99400.00 87700.00 73267.00 59847.00 47595.00 38848.00 30977.00 23629.00 15421.00 8688.00 3910.00 1307.00 451.00 Spots Measured (%): 0.60 0.02 0.03 0.07 0.15 0.27 0.47 0.80 1.30 2.01 2.85 3.72 4.69 5.50 6.32 7.20 8.06 9.12 9.48 8.36 6.99 5.71 4.54 3.70 2.95 2.25 1.47 0.83 0.37 0.12 0.04 Average thickness (Entire area) (µm): 16.93627 Average thickness (Biomass) (µm): 17.03769 Appended to file on: 2019-07-05 16:47:34 Image name: 11 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - 50% - C=0.ome.tif: B - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 Spots Measured (abs): 33916.00 2368.00 9454.00 33268.00 92031.00 175976.00 234621.00 220360.00 151860.00 69035.00 21311.00 3974.00 366.00 36.00 Spots Measured (%): 3.23 0.23 0.90 3.17 8.78 16.78 22.38 21.02 14.48 6.58 2.03 0.38 0.03 0.00 Average thickness (Entire area) (µm): 6.11809 Average thickness (Biomass) (µm): 6.32259 Appended to file on: 2019-07-05 16:47:35 Image name: 12 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - 50% - C=1.ome.tif: B - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 Spots Measured (abs): 403219.00 20506.00 32423.00 57428.00 89671.00 114796.00 116289.00 91535.00 60998.00 34701.00 17471.00 7012.00 2036.00 491.00 Spots Measured (%): 38.45 1.96 3.09 5.48 8.55 10.95 11.09 8.73 5.82 3.31 1.67 0.67 0.19 0.05 Average thickness (Entire area) (µm): 3.44338 Average thickness (Biomass) (µm): 5.59480 Appended to file on: 2019-07-05 16:47:36 Image name: 13 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - 75% - C=0.ome.tif: B - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 Spots Measured (abs): 400.00 30.00 70.00 325.00 1709.00 9938.00 39778.00 93815.00 149917.00 187570.00 188885.00 163323.00 107716.00 57682.00 24674.00 11139.00 6208.00 3351.00 1378.00 540.00 128.00 Spots Measured (%): 0.04 0.00 0.01 0.03 0.16 0.95 3.79 8.95 14.30 17.89 18.01 15.58 10.27 5.50 2.35 1.06 0.59 0.32 0.13 0.05 0.01 Average thickness (Entire area) (µm): 9.79588 Average thickness (Biomass) (µm): 9.79962 Appended to file on: 2019-07-05 16:47:37 Image name: 14 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - 75% - C=1.ome.tif: B - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 Spots Measured (abs): 91103.00 4932.00 7249.00 16788.00 36868.00 68777.00 106624.00 138068.00 148261.00 137859.00 112682.00 82223.00 49796.00 26043.00 11842.00 5252.00 2495.00 1034.00 451.00 165.00 64.00 Spots Measured (%): 8.69 0.47 0.69 1.60 3.52 6.56 10.17 13.17 14.14 13.15 10.75 7.84 4.75 2.48 1.13 0.50 0.24 0.10 0.04 0.02 0.01 Average thickness (Entire area) (µm): 7.50905 Average thickness (Biomass) (µm): 8.22353 Appended to file on: 2019-07-05 16:47:38 Image name: 15 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - Outlet - C=0.ome.tif: B - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 Spots Measured (abs): 29130.00 24.00 39.00 189.00 1088.00 4955.00 20435.00 62357.00 132978.00 202285.00 205009.00 162486.00 106325.00 64961.00 35280.00 15412.00 4787.00 836.00 Spots Measured (%): 2.78 0.00 0.00 0.02 0.10 0.47 1.95 5.95 12.68 19.29 19.55 15.50 10.14 6.20 3.36 1.47 0.46 0.08 Average thickness (Entire area) (µm): 9.76939 Average thickness (Biomass) (µm): 10.04855 Appended to file on: 2019-07-05 16:47:40 Image name: 16 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - Outlet - C=1.ome.tif: B - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 Spots Measured (abs): 470720.00 9214.00 6550.00 10994.00 20627.00 36077.00 56153.00 75616.00 87148.00 87117.00 71497.00 50903.00 32261.00 17826.00 9440.00 4502.00 1441.00 490.00 Spots Measured (%): 44.89 0.88 0.62 1.05 1.97 3.44 5.36 7.21 8.31 8.31 6.82 4.85 3.08 1.70 0.90 0.43 0.14 0.05 Average thickness (Entire area) (µm): 4.56721 Average thickness (Biomass) (µm): 8.28765 Appended to file on: 2019-07-05 16:47:41 Image name: 17 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - 50% - C=0.ome.tif: C - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 Spots Measured (abs): 5705.00 253.00 516.00 1460.00 3389.00 6832.00 14081.00 27398.00 48186.00 73109.00 93930.00 108328.00 118601.00 116251.00 106746.00 94427.00 78818.00 58451.00 38950.00 23771.00 14394.00 7816.00 4472.00 1874.00 612.00 206.00 Spots Measured (%): 0.54 0.02 0.05 0.14 0.32 0.65 1.34 2.61 4.60 6.97 8.96 10.33 11.31 11.09 10.18 9.01 7.52 5.57 3.71 2.27 1.37 0.75 0.43 0.18 0.06 0.02 Average thickness (Entire area) (µm): 12.74699 Average thickness (Biomass) (µm): 12.81672 Appended to file on: 2019-07-05 16:47:43 Image name: 18 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - 50% - C=1.ome.tif: C - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 Spots Measured (abs): 541163.00 10219.00 7838.00 11549.00 18196.00 25874.00 34425.00 42425.00 48297.00 49676.00 50067.00 46698.00 40619.00 33105.00 26446.00 20697.00 15148.00 10158.00 6360.00 3998.00 2516.00 1550.00 928.00 407.00 126.00 91.00 Spots Measured (%): 51.61 0.97 0.75 1.10 1.74 2.47 3.28 4.05 4.61 4.74 4.77 4.45 3.87 3.16 2.52 1.97 1.44 0.97 0.61 0.38 0.24 0.15 0.09 0.04 0.01 0.01 Average thickness (Entire area) (µm): 4.70589 Average thickness (Biomass) (µm): 9.72478 Appended to file on: 2019-07-05 16:47:45 Image name: 19 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - 75% - C=0.ome.tif: C - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 Spots Measured (abs): 7.00 3.00 2.00 14.00 86.00 413.00 1861.00 6874.00 20091.00 44669.00 73936.00 117662.00 137928.00 160128.00 160046.00 140182.00 93776.00 51352.00 24112.00 10715.00 3592.00 894.00 197.00 36.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.01 0.04 0.18 0.66 1.92 4.26 7.05 11.22 13.15 15.27 15.26 13.37 8.94 4.90 2.30 1.02 0.34 0.09 0.02 0.00 0.00 Average thickness (Entire area) (µm): 13.19974 Average thickness (Biomass) (µm): 13.19983 Appended to file on: 2019-07-05 16:47:47 Image name: 2 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - Inlet - C=1.ome.tif: A - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 Spots Measured (abs): 302768.00 3133.00 2814.00 7672.00 20406.00 36937.00 49284.00 55370.00 58657.00 57379.00 44609.00 36357.00 32551.00 28433.00 25954.00 24573.00 23385.00 23047.00 24774.00 24489.00 23832.00 22404.00 21148.00 20043.00 17903.00 15663.00 12883.00 10103.00 7564.00 5268.00 3661.00 2286.00 1538.00 1027.00 661.00 Spots Measured (%): 28.87 0.30 0.27 0.73 1.95 3.52 4.70 5.28 5.59 5.47 4.25 3.47 3.10 2.71 2.48 2.34 2.23 2.20 2.36 2.34 2.27 2.14 2.02 1.91 1.71 1.49 1.23 0.96 0.72 0.50 0.35 0.22 0.15 0.10 0.06 Average thickness (Entire area) (µm): 9.47252 Average thickness (Biomass) (µm): 13.31798 Appended to file on: 2019-07-05 16:47:49 Image name: 20 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - 75% - C=1.ome.tif: C - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 Spots Measured (abs): 30956.00 1348.00 1742.00 3670.00 7868.00 15554.00 26861.00 42635.00 62750.00 85100.00 105295.00 122302.00 126514.00 123229.00 107274.00 83801.00 51617.00 27309.00 12778.00 5848.00 2383.00 1152.00 429.00 130.00 31.00 Spots Measured (%): 2.95 0.13 0.17 0.35 0.75 1.48 2.56 4.07 5.98 8.12 10.04 11.66 12.07 11.75 10.23 7.99 4.92 2.60 1.22 0.56 0.23 0.11 0.04 0.01 0.00 Average thickness (Entire area) (µm): 11.29396 Average thickness (Biomass) (µm): 11.63752 Appended to file on: 2019-07-05 16:47:51 Image name: 21 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - 25% - C=0.ome.tif: C - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 84804.00 2711.00 3607.00 6694.00 11709.00 19757.00 30527.00 43958.00 56481.00 66495.00 69709.00 68008.00 62750.00 56629.00 52478.00 48978.00 48550.00 47207.00 46710.00 44997.00 40361.00 34774.00 29272.00 25689.00 21597.00 13916.00 7172.00 2485.00 439.00 97.00 12.00 3.00 Spots Measured (%): 8.09 0.26 0.34 0.64 1.12 1.88 2.91 4.19 5.39 6.34 6.65 6.49 5.98 5.40 5.00 4.67 4.63 4.50 4.45 4.29 3.85 3.32 2.79 2.45 2.06 1.33 0.68 0.24 0.04 0.01 0.00 0.00 Average thickness (Entire area) (µm): 12.68313 Average thickness (Biomass) (µm): 13.79914 Appended to file on: 2019-07-05 16:47:52 Image name: 22 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - 25% - C=1.ome.tif: C - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 382248.00 9534.00 8794.00 11415.00 16266.00 21147.00 26978.00 33306.00 40249.00 45066.00 47230.00 45450.00 42051.00 38821.00 34812.00 30961.00 29272.00 27254.00 26343.00 24467.00 21851.00 19499.00 16386.00 14407.00 11796.00 8783.00 6394.00 4109.00 1976.00 987.00 448.00 276.00 Spots Measured (%): 36.45 0.91 0.84 1.09 1.55 2.02 2.57 3.18 3.84 4.30 4.50 4.33 4.01 3.70 3.32 2.95 2.79 2.60 2.51 2.33 2.08 1.86 1.56 1.37 1.12 0.84 0.61 0.39 0.19 0.09 0.04 0.03 Average thickness (Entire area) (µm): 8.18289 Average thickness (Biomass) (µm): 12.87712 Appended to file on: 2019-07-05 16:47:53 Image name: 23 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - Inlet - C=0.ome.tif: C - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 Spots Measured (abs): 4285.00 239.00 1059.00 4279.00 11532.00 27208.00 56947.00 98834.00 130035.00 147389.00 136885.00 106638.00 81304.00 65495.00 53024.00 43968.00 31966.00 23283.00 14886.00 6919.00 2035.00 289.00 66.00 11.00 Spots Measured (%): 0.41 0.02 0.10 0.41 1.10 2.59 5.43 9.43 12.40 14.06 13.05 10.17 7.75 6.25 5.06 4.19 3.05 2.22 1.42 0.66 0.19 0.03 0.01 0.00 Average thickness (Entire area) (µm): 10.22377 Average thickness (Biomass) (µm): 10.26572 Appended to file on: 2019-07-05 16:47:55 Image name: 24 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - Inlet - C=1.ome.tif: C - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 Spots Measured (abs): 183664.00 2686.00 3807.00 8264.00 16023.00 27300.00 42666.00 63097.00 85983.00 106929.00 114796.00 103065.00 82645.00 62340.00 45132.00 33061.00 23578.00 16556.00 10926.00 6990.00 4281.00 2528.00 1438.00 821.00 Spots Measured (%): 17.52 0.26 0.36 0.79 1.53 2.60 4.07 6.02 8.20 10.20 10.95 9.83 7.88 5.95 4.30 3.15 2.25 1.58 1.04 0.67 0.41 0.24 0.14 0.08 Average thickness (Entire area) (µm): 8.55436 Average thickness (Biomass) (µm): 10.37088 Appended to file on: 2019-07-05 16:47:57 Image name: 25 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - 25% - C=0.ome.tif: B - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 Spots Measured (abs): 373.00 39.00 111.00 301.00 1321.00 6326.00 29343.00 99250.00 206722.00 265608.00 223561.00 136446.00 58158.00 17023.00 3474.00 496.00 24.00 Spots Measured (%): 0.04 0.00 0.01 0.03 0.13 0.60 2.80 9.47 19.71 25.33 21.32 13.01 5.55 1.62 0.33 0.05 0.00 Average thickness (Entire area) (µm): 9.21479 Average thickness (Biomass) (µm): 9.21807 Appended to file on: 2019-07-05 16:47:58 Image name: 26 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - 25% - C=1.ome.tif: B - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 Spots Measured (abs): 87930.00 4724.00 4413.00 8644.00 23161.00 55213.00 103382.00 158788.00 192032.00 180242.00 126736.00 65917.00 26975.00 8222.00 1864.00 292.00 41.00 Spots Measured (%): 8.39 0.45 0.42 0.82 2.21 5.27 9.86 15.14 18.31 17.19 12.09 6.29 2.57 0.78 0.18 0.03 0.00 Average thickness (Entire area) (µm): 7.39124 Average thickness (Biomass) (µm): 8.06777 Appended to file on: 2019-07-05 16:47:59 Image name: 27 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - Inlet - C=0.ome.tif: B - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 Spots Measured (abs): 288796.00 10657.00 20002.00 42424.00 68621.00 96737.00 111267.00 117869.00 108472.00 85016.00 57276.00 26909.00 10420.00 4110.00 Spots Measured (%): 27.54 1.02 1.91 4.05 6.54 9.23 10.61 11.24 10.34 8.11 5.46 2.57 0.99 0.39 Average thickness (Entire area) (µm): 4.87080 Average thickness (Biomass) (µm): 6.72221 Appended to file on: 2019-07-05 16:48:00 Image name: 28 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - B - Inlet - C=1.ome.tif: B - Inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 Spots Measured (abs): 355457.00 23450.00 29864.00 54934.00 86997.00 112818.00 117658.00 104392.00 77072.00 46175.00 24623.00 9938.00 3791.00 1407.00 Spots Measured (%): 33.90 2.24 2.85 5.24 8.30 10.76 11.22 9.96 7.35 4.40 2.35 0.95 0.36 0.13 Average thickness (Entire area) (µm): 3.85954 Average thickness (Biomass) (µm): 5.83885 Appended to file on: 2019-07-05 16:48:02 Image name: 29 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - Outlet - C=0.ome.tif: C - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 0.00 0.00 0.00 3.00 20.00 68.00 331.00 1305.00 3645.00 8335.00 16894.00 30268.00 47668.00 64109.00 73400.00 82869.00 94198.00 104049.00 105054.00 100336.00 88381.00 71343.00 53547.00 38861.00 26879.00 16303.00 8796.00 4590.00 2876.00 2193.00 1469.00 786.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.12 0.35 0.79 1.61 2.89 4.55 6.11 7.00 7.90 8.98 9.92 10.02 9.57 8.43 6.80 5.11 3.71 2.56 1.55 0.84 0.44 0.27 0.21 0.14 0.07 Average thickness (Entire area) (µm): 17.46851 Average thickness (Biomass) (µm): 17.46851 Appended to file on: 2019-07-05 16:48:04 Image name: 3 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - 25% - C=0.ome.tif: A - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 Spots Measured (abs): 2695.00 96.00 256.00 756.00 2372.00 6400.00 11319.00 14617.00 15399.00 14732.00 14646.00 14197.00 13281.00 12231.00 11753.00 10745.00 10927.00 11563.00 12758.00 14186.00 16462.00 18847.00 20779.00 22802.00 24155.00 26361.00 29880.00 35524.00 39203.00 39760.00 38807.00 41651.00 50065.00 67576.00 51803.00 59634.00 66906.00 69371.00 58562.00 38386.00 22295.00 10158.00 4660.00 Spots Measured (%): 0.26 0.01 0.02 0.07 0.23 0.61 1.08 1.39 1.47 1.40 1.40 1.35 1.27 1.17 1.12 1.02 1.04 1.10 1.22 1.35 1.57 1.80 1.98 2.17 2.30 2.51 2.85 3.39 3.74 3.79 3.70 3.97 4.77 6.44 4.94 5.69 6.38 6.62 5.58 3.66 2.13 0.97 0.44 Average thickness (Entire area) (µm): 28.17978 Average thickness (Biomass) (µm): 28.25239 Appended to file on: 2019-07-05 16:48:06 Image name: 30 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - C - Outlet - C=1.ome.tif: C - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 Spots Measured (abs): 35335.00 757.00 621.00 1035.00 1948.00 3905.00 6723.00 11459.00 17857.00 27254.00 37598.00 50232.00 62665.00 73304.00 80236.00 84881.00 88254.00 88017.00 84544.00 75607.00 62385.00 48694.00 36679.00 25584.00 16887.00 10386.00 6345.00 3909.00 2537.00 1598.00 825.00 515.00 Spots Measured (%): 3.37 0.07 0.06 0.10 0.19 0.37 0.64 1.09 1.70 2.60 3.59 4.79 5.98 6.99 7.65 8.09 8.42 8.39 8.06 7.21 5.95 4.64 3.50 2.44 1.61 0.99 0.61 0.37 0.24 0.15 0.08 0.05 Average thickness (Entire area) (µm): 15.44199 Average thickness (Biomass) (µm): 15.98051 Appended to file on: 2019-07-05 16:48:08 Image name: 4 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - 25% - C=1.ome.tif: A - 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 37.99 38.99 39.99 40.99 41.99 Spots Measured (abs): 106597.00 1838.00 1666.00 3215.00 5922.00 9929.00 14398.00 17146.00 17260.00 16061.00 14919.00 13697.00 12547.00 11705.00 11162.00 10825.00 11072.00 11628.00 12223.00 12684.00 14065.00 16373.00 18631.00 21458.00 23744.00 26170.00 28418.00 31249.00 32367.00 34287.00 37331.00 44748.00 52125.00 64725.00 56773.00 59683.00 56055.00 45744.00 32164.00 18862.00 9958.00 4824.00 2328.00 Spots Measured (%): 10.17 0.18 0.16 0.31 0.56 0.95 1.37 1.64 1.65 1.53 1.42 1.31 1.20 1.12 1.06 1.03 1.06 1.11 1.17 1.21 1.34 1.56 1.78 2.05 2.26 2.50 2.71 2.98 3.09 3.27 3.56 4.27 4.97 6.17 5.41 5.69 5.35 4.36 3.07 1.80 0.95 0.46 0.22 Average thickness (Entire area) (µm): 24.08503 Average thickness (Biomass) (µm): 26.81056 Appended to file on: 2019-07-05 16:48:10 Image name: 5 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - 50% - C=0.ome.tif: A - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 Spots Measured (abs): 28168.00 1792.00 2915.00 5492.00 9698.00 15688.00 20342.00 21295.00 21359.00 21331.00 20862.00 19249.00 17780.00 18458.00 20128.00 23032.00 25838.00 29254.00 35684.00 43691.00 54185.00 57374.00 62537.00 69772.00 76389.00 82373.00 71731.00 51395.00 40583.00 29513.00 20965.00 13274.00 8179.00 4974.00 2627.00 593.00 56.00 Spots Measured (%): 2.69 0.17 0.28 0.52 0.92 1.50 1.94 2.03 2.04 2.03 1.99 1.84 1.70 1.76 1.92 2.20 2.46 2.79 3.40 4.17 5.17 5.47 5.96 6.65 7.29 7.86 6.84 4.90 3.87 2.81 2.00 1.27 0.78 0.47 0.25 0.06 0.01 Average thickness (Entire area) (µm): 19.68219 Average thickness (Biomass) (µm): 20.22551 Appended to file on: 2019-07-05 16:48:12 Image name: 6 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - 50% - C=1.ome.tif: A - 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 Spots Measured (abs): 226666.00 13925.00 11522.00 12340.00 14113.00 16310.00 17984.00 18707.00 17710.00 15924.00 14954.00 13769.00 13931.00 15248.00 17868.00 21787.00 26395.00 32593.00 39571.00 47028.00 52157.00 54873.00 55462.00 54976.00 51277.00 45426.00 37909.00 29927.00 21982.00 14601.00 9642.00 5836.00 3249.00 1682.00 759.00 297.00 176.00 Spots Measured (%): 21.62 1.33 1.10 1.18 1.35 1.56 1.72 1.78 1.69 1.52 1.43 1.31 1.33 1.45 1.70 2.08 2.52 3.11 3.77 4.48 4.97 5.23 5.29 5.24 4.89 4.33 3.62 2.85 2.10 1.39 0.92 0.56 0.31 0.16 0.07 0.03 0.02 Average thickness (Entire area) (µm): 14.40814 Average thickness (Biomass) (µm): 18.38161 Appended to file on: 2019-07-05 16:48:14 Image name: 7 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - 75% - C=0.ome.tif: A - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 Spots Measured (abs): 2655.00 273.00 556.00 1097.00 1983.00 2784.00 3439.00 3789.00 3402.00 2993.00 2949.00 3234.00 3615.00 4082.00 4747.00 5335.00 6260.00 6779.00 9571.00 13267.00 17425.00 23922.00 33882.00 49514.00 66940.00 82681.00 97281.00 109715.00 119976.00 103378.00 85744.00 66832.00 48990.00 33789.00 19060.00 5805.00 758.00 74.00 Spots Measured (%): 0.25 0.03 0.05 0.10 0.19 0.27 0.33 0.36 0.32 0.29 0.28 0.31 0.34 0.39 0.45 0.51 0.60 0.65 0.91 1.27 1.66 2.28 3.23 4.72 6.38 7.89 9.28 10.46 11.44 9.86 8.18 6.37 4.67 3.22 1.82 0.55 0.07 0.01 Average thickness (Entire area) (µm): 26.25748 Average thickness (Biomass) (µm): 26.32414 Appended to file on: 2019-07-05 16:48:16 Image name: 8 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - 75% - C=1.ome.tif: A - 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 30.99 31.99 32.99 33.99 34.99 35.99 36.99 Spots Measured (abs): 86573.00 4620.00 3948.00 3795.00 4095.00 4408.00 4277.00 4480.00 3720.00 3419.00 3051.00 2829.00 3105.00 3575.00 4629.00 6681.00 9866.00 14908.00 21911.00 31341.00 42774.00 55705.00 67285.00 77834.00 84319.00 87435.00 87422.00 83920.00 74832.00 57773.00 42944.00 28955.00 17146.00 9243.00 3952.00 1252.00 407.00 147.00 Spots Measured (%): 8.26 0.44 0.38 0.36 0.39 0.42 0.41 0.43 0.35 0.33 0.29 0.27 0.30 0.34 0.44 0.64 0.94 1.42 2.09 2.99 4.08 5.31 6.42 7.42 8.04 8.34 8.34 8.00 7.14 5.51 4.10 2.76 1.64 0.88 0.38 0.12 0.04 0.01 Average thickness (Entire area) (µm): 21.87465 Average thickness (Biomass) (µm): 23.84320 Appended to file on: 2019-07-05 16:48:17 Image name: 9 - Ray Allan 72h PAO1 WT microfluidics 27.05.19.lif - A - Outlet - C=0.ome.tif: A - Outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 16.99 17.99 18.99 19.99 20.99 21.99 22.99 23.99 24.99 25.99 26.99 27.99 28.99 29.99 Spots Measured (abs): 5.00 0.00 0.00 1.00 6.00 54.00 284.00 1139.00 3155.00 8291.00 16057.00 24473.00 36372.00 45302.00 53993.00 62343.00 72981.00 89496.00 110154.00 108424.00 94421.00 78125.00 61244.00 50375.00 41852.00 37357.00 27320.00 15934.00 6821.00 1950.00 647.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.11 0.30 0.79 1.53 2.33 3.47 4.32 5.15 5.95 6.96 8.54 10.51 10.34 9.00 7.45 5.84 4.80 3.99 3.56 2.61 1.52 0.65 0.19 0.06 Average thickness (Entire area) (µm): 18.46608 Average thickness (Biomass) (µm): 18.46617