Thickness distribution File created on: 2019-07-25 13:03:46 Image name: 1 - 2019-07-01 70h biofilm.lif - FC1 Ch1 inlet - C=0.ome.tif: FC1 Ch1 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 33.00 34.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.00 65.00 662.00 3575.00 12405.00 32238.00 69720.00 117537.00 152438.00 158186.00 141505.00 111878.00 86960.00 63271.00 41548.00 26847.00 16069.00 8082.00 3630.00 1317.00 459.00 126.00 52.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.06 0.34 1.18 3.07 6.65 11.21 14.54 15.09 13.49 10.67 8.29 6.03 3.96 2.56 1.53 0.77 0.35 0.13 0.04 0.01 0.00 Average thickness (Entire area) (µm): 21.63165 Average thickness (Biomass) (µm): 21.63165 Appended to file on: 2019-07-25 13:03:48 Image name: 10 - 2019-07-01 70h biofilm.lif - FC1 Ch1 75% - C=0.ome.tif: FC1 Ch1 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 Spots Measured (abs): 23.00 17.00 56.00 202.00 754.00 2328.00 7760.00 23909.00 68472.00 156889.00 238565.00 235633.00 154857.00 78182.00 36334.00 15948.00 8345.00 6405.00 5391.00 4203.00 2345.00 1286.00 541.00 116.00 15.00 0.00 0.00 Spots Measured (%): 0.00 0.00 0.01 0.02 0.07 0.22 0.74 2.28 6.53 14.96 22.75 22.47 14.77 7.46 3.47 1.52 0.80 0.61 0.51 0.40 0.22 0.12 0.05 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 10.77326 Average thickness (Biomass) (µm): 10.77349 Appended to file on: 2019-07-25 13:03:49 Image name: 11 - 2019-07-01 70h biofilm.lif - FC1 Ch1 75% - C=1.ome.tif: FC1 Ch1 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 Spots Measured (abs): 627071.00 2122.00 5136.00 8593.00 8808.00 9606.00 12397.00 17752.00 30590.00 52008.00 59079.00 53532.00 50261.00 50289.00 34542.00 14556.00 4566.00 2524.00 2291.00 1521.00 867.00 272.00 120.00 51.00 22.00 0.00 0.00 Spots Measured (%): 59.80 0.20 0.49 0.82 0.84 0.92 1.18 1.69 2.92 4.96 5.63 5.11 4.79 4.80 3.29 1.39 0.44 0.24 0.22 0.15 0.08 0.03 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 4.18140 Average thickness (Biomass) (µm): 10.40204 Appended to file on: 2019-07-25 13:03:51 Image name: 13 - 2019-07-01 70h biofilm.lif - FC1 Ch1 outlet - C=0.ome.tif: FC1 Ch1 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 21.00 367.00 2668.00 11616.00 33342.00 68910.00 110943.00 145625.00 155980.00 147609.00 123026.00 90079.00 63267.00 40448.00 25476.00 16937.00 12261.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.25 1.11 3.18 6.57 10.58 13.89 14.88 14.08 11.73 8.59 6.03 3.86 2.43 1.62 1.17 Average thickness (Entire area) (µm): 20.67016 Average thickness (Biomass) (µm): 20.67016 Appended to file on: 2019-07-25 13:03:54 Image name: 14 - 2019-07-01 70h biofilm.lif - FC1 Ch1 outlet - C=1.ome.tif: FC1 Ch1 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.00 33.00 359.00 2273.00 7814.00 19622.00 38965.00 67982.00 103613.00 134804.00 149640.00 143146.00 116528.00 90627.00 67653.00 47684.00 31591.00 26236.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.22 0.75 1.87 3.72 6.48 9.88 12.86 14.27 13.65 11.11 8.64 6.45 4.55 3.01 2.50 Average thickness (Entire area) (µm): 21.63202 Average thickness (Biomass) (µm): 21.63202 Appended to file on: 2019-07-25 13:03:56 Image name: 16 - 2019-07-01 70h biofilm.lif - FC1 Ch2 inlet - C=0.ome.tif: FC1 Ch2 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 12.00 205.00 3084.00 19508.00 57739.00 108763.00 153970.00 160263.00 134410.00 107795.00 84502.00 65676.00 52240.00 38227.00 24488.00 15167.00 9372.00 5202.00 3224.00 1748.00 1722.00 1084.00 170.00 5.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.29 1.86 5.51 10.37 14.68 15.28 12.82 10.28 8.06 6.26 4.98 3.65 2.34 1.45 0.89 0.50 0.31 0.17 0.16 0.10 0.02 0.00 0.00 Average thickness (Entire area) (µm): 16.18430 Average thickness (Biomass) (µm): 16.18430 Appended to file on: 2019-07-25 13:03:58 Image name: 17 - 2019-07-01 70h biofilm.lif - FC1 Ch2 inlet - C=1.ome.tif: FC1 Ch2 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 1.00 1.00 3.00 15.00 37.00 117.00 330.00 935.00 2426.00 4808.00 8473.00 13991.00 25028.00 44554.00 77384.00 121136.00 155278.00 167593.00 152079.00 117087.00 76114.00 41933.00 21693.00 9901.00 4092.00 1799.00 815.00 477.00 476.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.03 0.09 0.23 0.46 0.81 1.33 2.39 4.25 7.38 11.55 14.81 15.98 14.50 11.17 7.26 4.00 2.07 0.94 0.39 0.17 0.08 0.05 0.05 Average thickness (Entire area) (µm): 20.86386 Average thickness (Biomass) (µm): 20.86386 Appended to file on: 2019-07-25 13:04:00 Image name: 19 - 2019-07-01 70h biofilm.lif - FC1 Ch2 25% - C=0.ome.tif: FC1 Ch2 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 35.00 6.00 63.00 281.00 1177.00 5145.00 19474.00 62888.00 150764.00 243423.00 240510.00 158168.00 80558.00 38253.00 20989.00 11173.00 5820.00 3441.00 2623.00 1665.00 1433.00 448.00 183.00 56.00 0.00 0.00 Spots Measured (%): 0.00 0.00 0.01 0.03 0.11 0.49 1.86 6.00 14.38 23.21 22.94 15.08 7.68 3.65 2.00 1.07 0.56 0.33 0.25 0.16 0.14 0.04 0.02 0.01 0.00 0.00 Average thickness (Entire area) (µm): 9.85038 Average thickness (Biomass) (µm): 9.85071 Appended to file on: 2019-07-25 13:04:02 Image name: 2 - 2019-07-01 70h biofilm.lif - FC1 Ch1 inlet - C=1.ome.tif: FC1 Ch1 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 33.00 34.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 1.00 21.00 122.00 434.00 1516.00 3832.00 7126.00 11142.00 14984.00 20266.00 29315.00 45497.00 70347.00 101381.00 130407.00 145473.00 142057.00 121501.00 90958.00 58964.00 33876.00 19355.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.04 0.14 0.37 0.68 1.06 1.43 1.93 2.80 4.34 6.71 9.67 12.44 13.87 13.55 11.59 8.67 5.62 3.23 1.85 Average thickness (Entire area) (µm): 27.86893 Average thickness (Biomass) (µm): 27.86893 Appended to file on: 2019-07-25 13:04:04 Image name: 20 - 2019-07-01 70h biofilm.lif - FC1 Ch2 25% - C=1.ome.tif: FC1 Ch2 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 370770.00 4396.00 7273.00 10326.00 14686.00 19729.00 28457.00 41423.00 60068.00 81322.00 94222.00 96502.00 86912.00 64208.00 37165.00 17263.00 7306.00 2738.00 1397.00 1178.00 883.00 250.00 87.00 15.00 0.00 0.00 Spots Measured (%): 35.36 0.42 0.69 0.98 1.40 1.88 2.71 3.95 5.73 7.76 8.99 9.20 8.29 6.12 3.54 1.65 0.70 0.26 0.13 0.11 0.08 0.02 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 6.46282 Average thickness (Biomass) (µm): 9.99807 Appended to file on: 2019-07-25 13:04:05 Image name: 22 - 2019-07-01 70h biofilm.lif - FC1 Ch2 50% - C=0.ome.tif: FC1 Ch2 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 15.00 3.00 6.00 55.00 340.00 2100.00 12393.00 52421.00 151399.00 266779.00 280521.00 177206.00 58939.00 19193.00 8804.00 5992.00 5060.00 3946.00 2469.00 769.00 132.00 17.00 17.00 0.00 0.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.01 0.03 0.20 1.18 5.00 14.44 25.44 26.75 16.90 5.62 1.83 0.84 0.57 0.48 0.38 0.24 0.07 0.01 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 9.72717 Average thickness (Biomass) (µm): 9.72730 Appended to file on: 2019-07-25 13:04:07 Image name: 23 - 2019-07-01 70h biofilm.lif - FC1 Ch2 50% - C=1.ome.tif: FC1 Ch2 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 188221.00 2533.00 5332.00 8465.00 11964.00 17635.00 27856.00 48469.00 79520.00 110254.00 128263.00 131400.00 117948.00 84546.00 46273.00 21235.00 9772.00 5368.00 2491.00 799.00 168.00 44.00 19.00 1.00 0.00 0.00 Spots Measured (%): 17.95 0.24 0.51 0.81 1.14 1.68 2.66 4.62 7.58 10.51 12.23 12.53 11.25 8.06 4.41 2.03 0.93 0.51 0.24 0.08 0.02 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 8.42100 Average thickness (Biomass) (µm): 10.26327 Appended to file on: 2019-07-25 13:04:09 Image name: 25 - 2019-07-01 70h biofilm.lif - FC1 Ch2 75% - C=0.ome.tif: FC1 Ch2 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 Spots Measured (abs): 209.00 14.00 83.00 374.00 1290.00 4280.00 14064.00 49327.00 142553.00 257130.00 281253.00 176352.00 66217.00 25628.00 11111.00 4659.00 2709.00 2658.00 1990.00 1633.00 1446.00 822.00 457.00 436.00 370.00 663.00 705.00 134.00 9.00 0.00 0.00 0.00 Spots Measured (%): 0.02 0.00 0.01 0.04 0.12 0.41 1.34 4.70 13.59 24.52 26.82 16.82 6.31 2.44 1.06 0.44 0.26 0.25 0.19 0.16 0.14 0.08 0.04 0.04 0.04 0.06 0.07 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 9.80978 Average thickness (Biomass) (µm): 9.81173 Appended to file on: 2019-07-25 13:04:10 Image name: 26 - 2019-07-01 70h biofilm.lif - FC1 Ch2 75% - C=1.ome.tif: FC1 Ch2 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 Spots Measured (abs): 144711.00 3189.00 6879.00 10166.00 13560.00 17812.00 26082.00 41303.00 72723.00 106178.00 127916.00 136328.00 131594.00 105052.00 58812.00 24959.00 8642.00 3938.00 2108.00 1817.00 1110.00 735.00 484.00 447.00 679.00 645.00 550.00 157.00 0.00 0.00 0.00 0.00 Spots Measured (%): 13.80 0.30 0.66 0.97 1.29 1.70 2.49 3.94 6.94 10.13 12.20 13.00 12.55 10.02 5.61 2.38 0.82 0.38 0.20 0.17 0.11 0.07 0.05 0.04 0.06 0.06 0.05 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 9.04236 Average thickness (Biomass) (µm): 10.49006 Appended to file on: 2019-07-25 13:04:12 Image name: 28 - 2019-07-01 70h biofilm.lif - FC1 Ch2 outlet - C=0.ome.tif: FC1 Ch2 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 Spots Measured (abs): 3.00 0.00 0.00 0.00 2.00 10.00 48.00 293.00 1905.00 11836.00 49535.00 127224.00 187273.00 157870.00 118080.00 103665.00 89828.00 73088.00 52646.00 34282.00 21528.00 10796.00 5603.00 1981.00 716.00 253.00 111.00 0.00 0.00 0.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.18 1.13 4.72 12.13 17.86 15.06 11.26 9.89 8.57 6.97 5.02 3.27 2.05 1.03 0.53 0.19 0.07 0.02 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 13.97785 Average thickness (Biomass) (µm): 13.97789 Appended to file on: 2019-07-25 13:04:14 Image name: 29 - 2019-07-01 70h biofilm.lif - FC1 Ch2 outlet - C=1.ome.tif: FC1 Ch2 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 Spots Measured (abs): 56257.00 2538.00 3931.00 5264.00 5599.00 7310.00 9586.00 12790.00 18562.00 29290.00 47454.00 69413.00 87266.00 99042.00 115266.00 128663.00 116553.00 88800.00 60319.00 38800.00 23154.00 12745.00 6641.00 2064.00 747.00 424.00 97.00 1.00 0.00 0.00 Spots Measured (%): 5.37 0.24 0.37 0.50 0.53 0.70 0.91 1.22 1.77 2.79 4.53 6.62 8.32 9.45 10.99 12.27 11.12 8.47 5.75 3.70 2.21 1.22 0.63 0.20 0.07 0.04 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 13.26804 Average thickness (Biomass) (µm): 14.02024 Appended to file on: 2019-07-25 13:04:16 Image name: 31 - 2019-07-01 70h biofilm.lif - FC1 Ch3 inlet - C=0.ome.tif: FC1 Ch3 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 33.00 34.00 35.00 36.00 37.00 38.00 39.00 40.00 41.00 42.00 43.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 22.00 674.00 7589.00 37348.00 89730.00 130963.00 136276.00 110592.00 82292.00 65948.00 54799.00 44958.00 37067.00 29646.00 22614.00 18107.00 16349.00 15641.00 15189.00 14891.00 13988.00 12633.00 11360.00 11553.00 12991.00 16872.00 38484.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.06 0.72 3.56 8.56 12.49 13.00 10.55 7.85 6.29 5.23 4.29 3.53 2.83 2.16 1.73 1.56 1.49 1.45 1.42 1.33 1.20 1.08 1.10 1.24 1.61 3.67 Average thickness (Entire area) (µm): 26.84467 Average thickness (Biomass) (µm): 26.84467 Appended to file on: 2019-07-25 13:04:19 Image name: 32 - 2019-07-01 70h biofilm.lif - FC1 Ch3 inlet - C=1.ome.tif: FC1 Ch3 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 33.00 34.00 35.00 36.00 37.00 38.00 39.00 40.00 41.00 42.00 43.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 9.00 45.00 86.00 256.00 565.00 1313.00 2485.00 4302.00 6795.00 10552.00 17085.00 28523.00 44112.00 64094.00 83748.00 102497.00 118262.00 115243.00 105511.00 87766.00 69027.00 50700.00 36415.00 26568.00 19457.00 15182.00 13075.00 11459.00 13443.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.02 0.05 0.13 0.24 0.41 0.65 1.01 1.63 2.72 4.21 6.11 7.99 9.77 11.28 10.99 10.06 8.37 6.58 4.84 3.47 2.53 1.86 1.45 1.25 1.09 1.28 Average thickness (Entire area) (µm): 32.07708 Average thickness (Biomass) (µm): 32.07708 Appended to file on: 2019-07-25 13:04:21 Image name: 34 - 2019-07-01 70h biofilm.lif - FC1 Ch3 25% - C=0.ome.tif: FC1 Ch3 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 Spots Measured (abs): 82211.00 629.00 1102.00 1750.00 2536.00 4306.00 7438.00 11886.00 19543.00 31305.00 50184.00 85342.00 140026.00 179390.00 150742.00 97853.00 49809.00 33309.00 24819.00 21293.00 17087.00 9513.00 5699.00 3163.00 2741.00 2996.00 3808.00 3753.00 2954.00 1313.00 76.00 0.00 0.00 Spots Measured (%): 7.84 0.06 0.11 0.17 0.24 0.41 0.71 1.13 1.86 2.99 4.79 8.14 13.35 17.11 14.38 9.33 4.75 3.18 2.37 2.03 1.63 0.91 0.54 0.30 0.26 0.29 0.36 0.36 0.28 0.13 0.01 0.00 0.00 Average thickness (Entire area) (µm): 12.44940 Average thickness (Biomass) (µm): 13.50850 Appended to file on: 2019-07-25 13:04:23 Image name: 35 - 2019-07-01 70h biofilm.lif - FC1 Ch3 25% - C=1.ome.tif: FC1 Ch3 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 Spots Measured (abs): 481934.00 10695.00 13974.00 16958.00 17956.00 18406.00 18341.00 19308.00 20135.00 21116.00 23968.00 30208.00 40278.00 48858.00 51065.00 50662.00 46309.00 37163.00 29289.00 18519.00 10788.00 5942.00 3878.00 3666.00 2723.00 2403.00 1953.00 1318.00 609.00 152.00 2.00 0.00 0.00 Spots Measured (%): 45.96 1.02 1.33 1.62 1.71 1.76 1.75 1.84 1.92 2.01 2.29 2.88 3.84 4.66 4.87 4.83 4.42 3.54 2.79 1.77 1.03 0.57 0.37 0.35 0.26 0.23 0.19 0.13 0.06 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 6.68503 Average thickness (Biomass) (µm): 12.37070 Appended to file on: 2019-07-25 13:04:24 Image name: 37 - 2019-07-01 70h biofilm.lif - FC1 Ch3 50% - C=0.ome.tif: FC1 Ch3 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 293523.00 2942.00 4712.00 6182.00 7160.00 9470.00 13267.00 19343.00 31538.00 54727.00 94636.00 140261.00 163723.00 123684.00 55001.00 19547.00 5021.00 1616.00 941.00 605.00 340.00 224.00 83.00 30.00 0.00 0.00 Spots Measured (%): 27.99 0.28 0.45 0.59 0.68 0.90 1.27 1.84 3.01 5.22 9.03 13.38 15.61 11.80 5.25 1.86 0.48 0.15 0.09 0.06 0.03 0.02 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 7.95501 Average thickness (Biomass) (µm): 11.04748 Appended to file on: 2019-07-25 13:04:25 Image name: 38 - 2019-07-01 70h biofilm.lif - FC1 Ch3 50% - C=1.ome.tif: FC1 Ch3 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 683939.00 14884.00 18786.00 22500.00 21885.00 19680.00 19237.00 19172.00 21084.00 24586.00 28164.00 32863.00 32715.00 27034.00 20879.00 16174.00 11308.00 6733.00 3842.00 1701.00 841.00 350.00 125.00 90.00 4.00 0.00 Spots Measured (%): 65.23 1.42 1.79 2.15 2.09 1.88 1.83 1.83 2.01 2.34 2.69 3.13 3.12 2.58 1.99 1.54 1.08 0.64 0.37 0.16 0.08 0.03 0.01 0.01 0.00 0.00 Average thickness (Entire area) (µm): 3.14069 Average thickness (Biomass) (µm): 9.03159 Appended to file on: 2019-07-25 13:04:27 Image name: 4 - 2019-07-01 70h biofilm.lif - FC1 Ch1 25% - C=0.ome.tif: FC1 Ch1 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 13.00 525.00 6726.00 36704.00 101107.00 183614.00 235971.00 215671.00 142402.00 69935.00 26099.00 10691.00 6362.00 4969.00 4009.00 2325.00 1107.00 309.00 37.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.05 0.64 3.50 9.64 17.51 22.50 20.57 13.58 6.67 2.49 1.02 0.61 0.47 0.38 0.22 0.11 0.03 0.00 Average thickness (Entire area) (µm): 15.45995 Average thickness (Biomass) (µm): 15.45995 Appended to file on: 2019-07-25 13:04:28 Image name: 40 - 2019-07-01 70h biofilm.lif - FC1 Ch3 75% - C=0.ome.tif: FC1 Ch3 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 Spots Measured (abs): 412424.00 1624.00 3349.00 5009.00 5449.00 5766.00 7932.00 13406.00 21138.00 36061.00 67850.00 108445.00 142888.00 122282.00 59584.00 23137.00 9353.00 2123.00 582.00 153.00 21.00 0.00 Spots Measured (%): 39.33 0.15 0.32 0.48 0.52 0.55 0.76 1.28 2.02 3.44 6.47 10.34 13.63 11.66 5.68 2.21 0.89 0.20 0.06 0.01 0.00 0.00 Average thickness (Entire area) (µm): 6.92848 Average thickness (Biomass) (µm): 11.42028 Appended to file on: 2019-07-25 13:04:30 Image name: 41 - 2019-07-01 70h biofilm.lif - FC1 Ch3 75% - C=1.ome.tif: FC1 Ch3 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 Spots Measured (abs): 515283.00 12504.00 19766.00 23776.00 23082.00 22155.00 23418.00 27155.00 26578.00 28974.00 35039.00 42984.00 48242.00 46332.00 43020.00 42348.00 37766.00 19877.00 7837.00 2195.00 229.00 16.00 Spots Measured (%): 49.14 1.19 1.89 2.27 2.20 2.11 2.23 2.59 2.53 2.76 3.34 4.10 4.60 4.42 4.10 4.04 3.60 1.90 0.75 0.21 0.02 0.00 Average thickness (Entire area) (µm): 5.24697 Average thickness (Biomass) (µm): 10.31674 Appended to file on: 2019-07-25 13:04:32 Image name: 43 - 2019-07-01 70h biofilm.lif - FC1 Ch3 outlet - C=0.ome.tif: FC1 Ch3 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 9.00 173.00 1876.00 11911.00 45119.00 99781.00 140309.00 155806.00 142268.00 107280.00 82021.00 62110.00 51400.00 44863.00 33583.00 23214.00 16839.00 12385.00 8824.00 5488.00 2516.00 801.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.18 1.14 4.30 9.52 13.38 14.86 13.57 10.23 7.82 5.92 4.90 4.28 3.20 2.21 1.61 1.18 0.84 0.52 0.24 0.08 Average thickness (Entire area) (µm): 19.66484 Average thickness (Biomass) (µm): 19.66484 Appended to file on: 2019-07-25 13:04:34 Image name: 44 - 2019-07-01 70h biofilm.lif - FC1 Ch3 outlet - C=1.ome.tif: FC1 Ch3 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4.00 16.00 69.00 392.00 1372.00 3884.00 9128.00 18884.00 37053.00 67573.00 101628.00 125967.00 131134.00 117920.00 98284.00 78002.00 61375.00 51271.00 40866.00 30037.00 24751.00 21793.00 27173.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.04 0.13 0.37 0.87 1.80 3.53 6.44 9.69 12.01 12.51 11.25 9.37 7.44 5.85 4.89 3.90 2.86 2.36 2.08 2.59 Average thickness (Entire area) (µm): 23.23786 Average thickness (Biomass) (µm): 23.23786 Appended to file on: 2019-07-25 13:04:36 Image name: 46 - 2019-07-01 70h biofilm.lif - FC2 Ch1 inlet - C=0.ome.tif: FC2 Ch1 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 786.00 160.00 522.00 1531.00 4022.00 9674.00 22704.00 48815.00 91788.00 144312.00 180069.00 177788.00 147736.00 100096.00 59432.00 32072.00 16307.00 7056.00 2593.00 896.00 96.00 81.00 34.00 6.00 0.00 0.00 Spots Measured (%): 0.07 0.02 0.05 0.15 0.38 0.92 2.17 4.66 8.75 13.76 17.17 16.96 14.09 9.55 5.67 3.06 1.56 0.67 0.25 0.09 0.01 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 10.65553 Average thickness (Biomass) (µm): 10.66352 Appended to file on: 2019-07-25 13:04:37 Image name: 47 - 2019-07-01 70h biofilm.lif - FC2 Ch1 inlet - C=1.ome.tif: FC2 Ch1 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 692027.00 9671.00 21415.00 28836.00 33476.00 37161.00 40960.00 39224.00 33379.00 25895.00 19621.00 15573.00 13636.00 12056.00 9368.00 7381.00 4538.00 2336.00 1129.00 600.00 137.00 88.00 55.00 14.00 0.00 0.00 Spots Measured (%): 66.00 0.92 2.04 2.75 3.19 3.54 3.91 3.74 3.18 2.47 1.87 1.49 1.30 1.15 0.89 0.70 0.43 0.22 0.11 0.06 0.01 0.01 0.01 0.00 0.00 0.00 Average thickness (Entire area) (µm): 2.44031 Average thickness (Biomass) (µm): 7.17672 Appended to file on: 2019-07-25 13:04:39 Image name: 49 - 2019-07-01 70h biofilm.lif - FC2 Ch1 25% - C=0.ome.tif: FC2 Ch1 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 Spots Measured (abs): 2399.00 60.00 214.00 596.00 1631.00 3940.00 9356.00 19464.00 37092.00 64743.00 99649.00 133461.00 164829.00 165774.00 142830.00 97565.00 53666.00 28094.00 15472.00 5797.00 1395.00 420.00 116.00 13.00 Spots Measured (%): 0.23 0.01 0.02 0.06 0.16 0.38 0.89 1.86 3.54 6.17 9.50 12.73 15.72 15.81 13.62 9.30 5.12 2.68 1.48 0.55 0.13 0.04 0.01 0.00 Average thickness (Entire area) (µm): 12.32577 Average thickness (Biomass) (µm): 12.35404 Appended to file on: 2019-07-25 13:04:41 Image name: 5 - 2019-07-01 70h biofilm.lif - FC1 Ch1 25% - C=1.ome.tif: FC1 Ch1 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.00 10.00 143.00 641.00 1835.00 4179.00 6970.00 11074.00 19792.00 37351.00 68122.00 111685.00 156972.00 183270.00 174341.00 130006.00 79951.00 42314.00 19918.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.06 0.17 0.40 0.66 1.06 1.89 3.56 6.50 10.65 14.97 17.48 16.63 12.40 7.62 4.04 1.90 Average thickness (Entire area) (µm): 21.93804 Average thickness (Biomass) (µm): 21.93804 Appended to file on: 2019-07-25 13:04:43 Image name: 50 - 2019-07-01 70h biofilm.lif - FC2 Ch1 25% - C=1.ome.tif: FC2 Ch1 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 Spots Measured (abs): 10896.00 334.00 824.00 1594.00 2741.00 4496.00 7027.00 10845.00 16333.00 23826.00 31769.00 40594.00 51010.00 65911.00 91339.00 124374.00 149145.00 146166.00 118340.00 79275.00 40090.00 18221.00 8574.00 4852.00 Spots Measured (%): 1.04 0.03 0.08 0.15 0.26 0.43 0.67 1.03 1.56 2.27 3.03 3.87 4.86 6.29 8.71 11.86 14.22 13.94 11.29 7.56 3.82 1.74 0.82 0.46 Average thickness (Entire area) (µm): 15.13666 Average thickness (Biomass) (µm): 15.29560 Appended to file on: 2019-07-25 13:04:44 Image name: 52 - 2019-07-01 70h biofilm.lif - FC2 Ch2 50% - C=0.ome.tif: FC2 Ch2 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 Spots Measured (abs): 153014.00 4301.00 7943.00 13330.00 22423.00 37704.00 62776.00 98197.00 136553.00 169649.00 149393.00 98449.00 48896.00 25758.00 12186.00 5613.00 2069.00 286.00 36.00 0.00 Spots Measured (%): 14.59 0.41 0.76 1.27 2.14 3.60 5.99 9.36 13.02 16.18 14.25 9.39 4.66 2.46 1.16 0.54 0.20 0.03 0.00 0.00 Average thickness (Entire area) (µm): 7.45107 Average thickness (Biomass) (µm): 8.72415 Appended to file on: 2019-07-25 13:04:45 Image name: 53 - 2019-07-01 70h biofilm.lif - FC2 Ch2 50% - C=1.ome.tif: FC2 Ch2 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 Spots Measured (abs): 949199.00 10939.00 15996.00 14938.00 11202.00 9244.00 8211.00 7118.00 6006.00 5151.00 4149.00 2748.00 1341.00 554.00 649.00 731.00 371.00 19.00 10.00 0.00 Spots Measured (%): 90.52 1.04 1.53 1.42 1.07 0.88 0.78 0.68 0.57 0.49 0.40 0.26 0.13 0.05 0.06 0.07 0.04 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.47087 Average thickness (Biomass) (µm): 4.96834 Appended to file on: 2019-07-25 13:04:47 Image name: 55 - 2019-07-01 70h biofilm.lif - FC2 Ch2 75% - C=0.ome.tif: FC2 Ch2 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 Spots Measured (abs): 2783.00 104.00 232.00 447.00 780.00 1408.00 2421.00 3973.00 7404.00 14903.00 31396.00 65001.00 117915.00 157075.00 157934.00 138527.00 117558.00 86431.00 67230.00 37007.00 16599.00 10673.00 6349.00 3206.00 916.00 232.00 63.00 9.00 Spots Measured (%): 0.27 0.01 0.02 0.04 0.07 0.13 0.23 0.38 0.71 1.42 2.99 6.20 11.25 14.98 15.06 13.21 11.21 8.24 6.41 3.53 1.58 1.02 0.61 0.31 0.09 0.02 0.01 0.00 Average thickness (Entire area) (µm): 14.38552 Average thickness (Biomass) (µm): 14.42381 Appended to file on: 2019-07-25 13:04:49 Image name: 56 - 2019-07-01 70h biofilm.lif - FC2 Ch2 75% - C=1.ome.tif: FC2 Ch2 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 Spots Measured (abs): 998578.00 5329.00 7976.00 9750.00 8786.00 5323.00 3051.00 2170.00 1734.00 1391.00 1170.00 702.00 419.00 336.00 373.00 196.00 276.00 331.00 264.00 293.00 87.00 41.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 95.23 0.51 0.76 0.93 0.84 0.51 0.29 0.21 0.17 0.13 0.11 0.07 0.04 0.03 0.04 0.02 0.03 0.03 0.03 0.03 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.22135 Average thickness (Biomass) (µm): 4.64225 Appended to file on: 2019-07-25 13:04:51 Image name: 7 - 2019-07-01 70h biofilm.lif - FC1 Ch1 50% - C=0.ome.tif: FC1 Ch1 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.00 167.00 4053.00 30255.00 90813.00 155306.00 188318.00 170484.00 120497.00 65963.00 37919.00 30888.00 27034.00 26861.00 25012.00 22727.00 18012.00 13234.00 8959.00 6304.00 3332.00 1452.00 709.00 271.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.39 2.89 8.66 14.81 17.96 16.26 11.49 6.29 3.62 2.95 2.58 2.56 2.39 2.17 1.72 1.26 0.85 0.60 0.32 0.14 0.07 0.03 Average thickness (Entire area) (µm): 15.73943 Average thickness (Biomass) (µm): 15.73943 Appended to file on: 2019-07-25 13:04:53 Image name: 79 - 2019-07-01 70h biofilm.lif - FC2 Ch3 inlet - C=0.ome.tif: FC2 Ch3 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 33.00 34.00 35.00 36.00 37.00 38.00 39.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.00 8.00 94.00 915.00 4980.00 15544.00 35669.00 61161.00 84517.00 102353.00 112120.00 120303.00 117249.00 99832.00 78339.00 57759.00 40519.00 28463.00 21694.00 16747.00 12733.00 12263.00 11566.00 13746.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.09 0.47 1.48 3.40 5.83 8.06 9.76 10.69 11.47 11.18 9.52 7.47 5.51 3.86 2.71 2.07 1.60 1.21 1.17 1.10 1.31 Average thickness (Entire area) (µm): 27.80083 Average thickness (Biomass) (µm): 27.80083 Appended to file on: 2019-07-25 13:04:55 Image name: 8 - 2019-07-01 70h biofilm.lif - FC1 Ch1 50% - C=1.ome.tif: FC1 Ch1 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.00 14.00 91.00 515.00 1844.00 4339.00 8694.00 15718.00 28741.00 48028.00 70097.00 90441.00 104815.00 113786.00 111912.00 106040.00 94180.00 76870.00 58891.00 43211.00 29229.00 19548.00 12487.00 9083.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.05 0.18 0.41 0.83 1.50 2.74 4.58 6.68 8.63 10.00 10.85 10.67 10.11 8.98 7.33 5.62 4.12 2.79 1.86 1.19 0.87 Average thickness (Entire area) (µm): 21.94000 Average thickness (Biomass) (µm): 21.94000 Appended to file on: 2019-07-25 13:04:58 Image name: 80 - 2019-07-01 70h biofilm.lif - FC2 Ch3 inlet - C=1.ome.tif: FC2 Ch3 inlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 31.00 32.00 33.00 34.00 35.00 36.00 37.00 38.00 39.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 5.00 13.00 54.00 113.00 198.00 322.00 531.00 798.00 1329.00 2649.00 6614.00 16928.00 37273.00 66846.00 100984.00 125886.00 135986.00 140547.00 126249.00 102798.00 75134.00 57493.00 49825.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.01 0.02 0.03 0.05 0.08 0.13 0.25 0.63 1.61 3.55 6.37 9.63 12.01 12.97 13.40 12.04 9.80 7.17 5.48 4.75 Average thickness (Entire area) (µm): 33.69995 Average thickness (Biomass) (µm): 33.69995 Appended to file on: 2019-07-25 13:05:00 Image name: 82 - 2019-07-01 70h biofilm.lif - FC2 Ch3 25% - C=0.ome.tif: FC2 Ch3 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 Spots Measured (abs): 3242.00 15.00 23.00 51.00 109.00 277.00 797.00 2830.00 11029.00 39044.00 104751.00 194739.00 262867.00 214311.00 118227.00 55244.00 20604.00 8762.00 4590.00 2726.00 1925.00 1195.00 637.00 327.00 254.00 Spots Measured (%): 0.31 0.00 0.00 0.00 0.01 0.03 0.08 0.27 1.05 3.72 9.99 18.57 25.07 20.44 11.28 5.27 1.96 0.84 0.44 0.26 0.18 0.11 0.06 0.03 0.02 Average thickness (Entire area) (µm): 12.19132 Average thickness (Biomass) (µm): 12.22913 Appended to file on: 2019-07-25 13:05:01 Image name: 83 - 2019-07-01 70h biofilm.lif - FC2 Ch3 25% - C=1.ome.tif: FC2 Ch3 25% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 Spots Measured (abs): 979937.00 1643.00 2912.00 4686.00 6795.00 9458.00 10348.00 7997.00 6762.00 5065.00 3933.00 2407.00 2056.00 1575.00 1046.00 864.00 412.00 309.00 248.00 82.00 30.00 11.00 0.00 0.00 0.00 Spots Measured (%): 93.45 0.16 0.28 0.45 0.65 0.90 0.99 0.76 0.64 0.48 0.38 0.23 0.20 0.15 0.10 0.08 0.04 0.03 0.02 0.01 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.44915 Average thickness (Biomass) (µm): 6.86148 Appended to file on: 2019-07-25 13:05:03 Image name: 85 - 2019-07-01 70h biofilm.lif - FC2 Ch3 50% - C=0.ome.tif: FC2 Ch3 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 285.00 6.00 4.00 9.00 14.00 23.00 96.00 411.00 1976.00 10672.00 48087.00 131913.00 235838.00 231912.00 191465.00 107575.00 45511.00 15475.00 8370.00 6238.00 5320.00 4796.00 1925.00 500.00 149.00 6.00 Spots Measured (%): 0.03 0.00 0.00 0.00 0.00 0.00 0.01 0.04 0.19 1.02 4.59 12.58 22.49 22.12 18.26 10.26 4.34 1.48 0.80 0.59 0.51 0.46 0.18 0.05 0.01 0.00 Average thickness (Entire area) (µm): 13.07547 Average thickness (Biomass) (µm): 13.07903 Appended to file on: 2019-07-25 13:05:04 Image name: 86 - 2019-07-01 70h biofilm.lif - FC2 Ch3 50% - C=1.ome.tif: FC2 Ch3 50% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 Spots Measured (abs): 973187.00 1063.00 1613.00 2574.00 4432.00 7736.00 11506.00 10669.00 8189.00 6703.00 5980.00 4332.00 3748.00 2570.00 1728.00 1006.00 771.00 506.00 208.00 53.00 2.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 92.81 0.10 0.15 0.25 0.42 0.74 1.10 1.02 0.78 0.64 0.57 0.41 0.36 0.25 0.16 0.10 0.07 0.05 0.02 0.01 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.56151 Average thickness (Biomass) (µm): 7.80994 Appended to file on: 2019-07-25 13:05:06 Image name: 88 - 2019-07-01 70h biofilm.lif - FC2 Ch3 75% - C=0.ome.tif: FC2 Ch3 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 Spots Measured (abs): 3766.00 122.00 155.00 222.00 495.00 1343.00 3664.00 10431.00 31958.00 86619.00 179307.00 244745.00 243361.00 35993.00 27607.00 29103.00 23632.00 20672.00 20538.00 21234.00 22629.00 15951.00 12841.00 10586.00 1540.00 62.00 0.00 0.00 0.00 0.00 Spots Measured (%): 0.36 0.01 0.01 0.02 0.05 0.13 0.35 0.99 3.05 8.26 17.10 23.34 23.21 3.43 2.63 2.78 2.25 1.97 1.96 2.03 2.16 1.52 1.22 1.01 0.15 0.01 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 12.10249 Average thickness (Biomass) (µm): 12.14611 Appended to file on: 2019-07-25 13:05:07 Image name: 89 - 2019-07-01 70h biofilm.lif - FC2 Ch3 75% - C=1.ome.tif: FC2 Ch3 75% ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 Spots Measured (abs): 955919.00 1578.00 2009.00 3524.00 6073.00 11217.00 17062.00 13911.00 9760.00 7254.00 5835.00 3974.00 2903.00 790.00 487.00 622.00 582.00 447.00 544.00 1086.00 1874.00 1087.00 38.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spots Measured (%): 91.16 0.15 0.19 0.34 0.58 1.07 1.63 1.33 0.93 0.69 0.56 0.38 0.28 0.08 0.05 0.06 0.06 0.04 0.05 0.10 0.18 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Average thickness (Entire area) (µm): 0.67845 Average thickness (Biomass) (µm): 7.67786 Appended to file on: 2019-07-25 13:05:09 Image name: 91 - 2019-07-01 70h biofilm.lif - FC2 Ch3 outlet - C=0.ome.tif: FC2 Ch3 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4.00 146.00 3058.00 24856.00 93699.00 199489.00 273182.00 218572.00 124515.00 64092.00 29178.00 11579.00 4292.00 1361.00 366.00 187.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.29 2.37 8.94 19.02 26.05 20.84 11.87 6.11 2.78 1.10 0.41 0.13 0.03 0.02 Average thickness (Entire area) (µm): 21.37987 Average thickness (Biomass) (µm): 21.37987 Appended to file on: 2019-07-25 13:05:12 Image name: 92 - 2019-07-01 70h biofilm.lif - FC2 Ch3 outlet - C=1.ome.tif: FC2 Ch3 outlet ch1 Layer: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Thickness (µm): 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00 27.00 28.00 29.00 30.00 Spots Measured (abs): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 0.00 1.00 1.00 1.00 7.00 25.00 93.00 262.00 664.00 1774.00 5784.00 19201.00 53691.00 111538.00 168992.00 205030.00 190925.00 155011.00 135575.00 Spots Measured (%): 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 0.02 0.06 0.17 0.55 1.83 5.12 10.64 16.12 19.55 18.21 14.78 12.93 Average thickness (Entire area) (µm): 27.21759 Average thickness (Biomass) (µm): 27.21759